diff --git a/README.md b/README.md
--- a/README.md
+++ b/README.md
@@ -1,28 +1,27 @@
 # B.subtilis-GFP
 
-GFP expression experiments in B.subtilis
+This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus
 
  | 
 ----------------- | ----------------- 
-**strain**        | ?
-**synonyms**      | ?
-**parents**       | ?
-**obtained from** | ?
-**owner**         | jtl
+**strain**        | _B. subtilis_ 168
+**synonyms**      | 168
+**parents**       | None
+**obtained from** | ICOS lab
+**owner**         | Jonathan Tellechea
 
 ### Genotype
 
-TODO
+_trpC2_, _BC659_
 
 ### Phenotype
 
-TODO
+Trp(-)
 
 ### Comments
 
-TODO
+Wild type strain used in this study. Barcode 659 present in barcoding site between _ybxH_ and _ybxI_ genes. 
 
 ### References
 
-- TODO
-
+1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
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