# HG changeset patch
# User Jonathan Tellechea <j.tellechea-luzardo@newcastle.ac.uk>
# Date 2019-07-03 15:16:36
# Node ID 00cabd8c81afa1e0b7127af0560cf275cece62e3
# Parent  a507a439092004b667d10462c22cf23f787b1ebc

168-GFP strain description.

diff --git a/README.md b/README.md
--- a/README.md
+++ b/README.md
@@ -2,26 +2,28 @@
 
 This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus
 
+
  | 
 ----------------- | ----------------- 
-**strain**        | _B. subtilis_ 168
-**synonyms**      | 168
-**parents**       | None
+**strain**        | _B. subtilis_ 168-GFP
+**synonyms**      | 168-GFP
+**parents**       | 168 wild type
 **obtained from** | ICOS lab
 **owner**         | Jonathan Tellechea
 
 ### Genotype
 
-_trpC2_, _BC659_
+_trpC2_, _BC207-ble_, _amyE::GFP-cat_
 
 ### Phenotype
 
-Trp(-)
+Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. 
 
 ### Comments
 
-Wild type strain used in this study. Barcode 659 present in barcoding site between _ybxH_ and _ybxI_ genes. 
+168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). 
 
 ### References
 
-1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
\ No newline at end of file
+1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
+2. 2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23.
\ No newline at end of file