# HG changeset patch # User Jonathan Tellechea # Date 2019-07-03 15:00:48 # Node ID 7b624806c6cb6e81c3d56275a728d76170074d06 # Parent 070c713ad448f95a07020bb2f3146365cffb0517 GenBank file of the integrative plasmid used to insert GFP in amyE locus. diff --git a/pgfp-rrnb.gb b/pgfp-rrnb.gb new file mode 100644 --- /dev/null +++ b/pgfp-rrnb.gb @@ -0,0 +1,174 @@ +LOCUS pGFP-rrnB 8009 bp ds-DNA circular 08-NOV-2018 +DEFINITION . +ACCESSION pGFP +SOURCE Unknown. + ORGANISM Unknown Unclassified. +FEATURES Location/Qualifiers + CDS 6345..7052 + /label="gfp' CDS" + /ApEinfo_revcolor=#d59687 + /ApEinfo_fwdcolor=#d59687 + misc_feature complement(7173..7259) + /label="ter misc_feature" + /ApEinfo_revcolor=#b4abac + /ApEinfo_fwdcolor=#b4abac + misc_feature complement(7078..7166) + /label="(null) misc_feature" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + CDS 199..1071 + /label="bla CDS" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + CDS complement(2077..2892) + /label="spc CDS" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + CDS complement(7375..7895) + /label="amyE' CDS" + /ApEinfo_revcolor=#ff9ccd + /ApEinfo_fwdcolor=#ff9ccd + CDS complement(5367..6029) + /label="cat CDS" + /ApEinfo_revcolor=#f58a5e + /ApEinfo_fwdcolor=#f58a5e + CDS complement(3968..5005) + /label="'amyE CDS" + /ApEinfo_revcolor=#f8d3a9 + /ApEinfo_fwdcolor=#f8d3a9 +ORIGIN + 1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata + 61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt + 121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa + 181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt + 241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa + 301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac + 361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt + 421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt + 481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat + 541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac + 601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg + 661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc + 721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa + 781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag + 841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct + 901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat + 961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa + 1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac + 1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc + 1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc + 1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg + 1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg + 1321 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca + 1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg + 1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg + 1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga + 1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac + 1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat + 1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc + 1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga + 1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct + 1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg + 1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat + 1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc + 2041 agaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga + 2101 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc + 2161 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa + 2221 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt + 2281 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat + 2341 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag + 2401 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac + 2461 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct + 2521 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact + 2581 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga + 2641 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac + 2701 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa + 2761 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat + 2821 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gttgattatg + 2881 ttcatataaa gtttatattg atactcaatt tacttaccct agattggaca tatacttaaa + 2941 ttactgttca ataaagctga ccgttagcgt ttaagtacat cctttcacaa tttgtctaca + 3001 gattaataat tattctttat tatacagatc gatcctctag acctaggcct taagatctga + 3061 tcatatgcat ccgcgggccc gggttaacgc gtaatccatg gatcaagaga caggatgagg + 3121 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggtgc cctgaatgaa + 3181 ctgcagaaag agctggtagt tggcgcactg ttcgaagaac tgccgatgtc cagtaagatt + 3241 cttactatgc tggttgaacc ggatgctggt aaagctactt gggttgctgc ttctacttat + 3301 ggtaccgata caactactgg tgaggaagtt aaaggagctc ttaaagaaat ccacttcagt + 3361 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg + 3421 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga + 3481 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct + 3541 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga + 3601 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgacta + 3661 agaaaatgcc gtcaaatccg ctcgccatga cttcactaac gatgcctttg aaaatcttca + 3721 agttcttttc tactaattca aggcgtgtct caccaggttt ttggtttgct ccggcgcaaa + 3781 tgcagacaat atcagcatcc ttgcagggta tgtttctctt tgatgtcttt ttgtttgtga + 3841 agtatttcac atttatattg tgcaacactt cacaaacttt tgcaagagaa aagttttgtc + 3901 tgatttatga acaaaaaaga aaccatcatt gatggtttct ttcggtaagt cccgtctagc + 3961 cttgccctca atggggaaga gaaccgctta agcccgagtc attatataaa ccatttagca + 4021 cgtaatcaaa gccaggctga ttctgaccgg gcacttgggc gctgccatta ttaaaaatca + 4081 cttttgcgtt ggttgtatcc gtgtccgcag gcagcgtcag cgtgtaaatt ccgtctgcat + 4141 ttttagtcat tggttttcca ggccaagatc cggtcaattc aattactcgg ctcccatcat + 4201 gtttatagat ataagcattt acctggctcc aatgattcgg attttgatag ccgatggttt + 4261 tggccgacgc tggatctctt ttaacaaaac tgtatttctc ggtcctcgtt acaccatcac + 4321 tgttcgttcc ttttaacatg atggtgtatg ttttgccaaa ttggatctcc ttttccgatt + 4381 gtgaattgat ctccatcctt aaacgcctgt cgtctggtcc attattgatt tgataaacgg + 4441 cttttgttgt attcgcatct gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg + 4501 ttacacctgt tttgtaattc tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat + 4561 aaagcacagc tacagacctg gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa + 4621 atgaacccgc tccagcttta ttgtcatacc tgccatcagg caattttgtt gccgtattga + 4681 tagagacaga ggatgaacct gcatttgcca gcacaacgcc atgtgagccg cgctgattca + 4741 taaatatctg gttgtttcca ttcgggttcg agagttcctc aggctgtcca gccatcacat + 4801 tgtgaaatct attgaccgca gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc + 4861 ctatttggct tttccccggg aacctcacac catttccgcc tccctcaggt ctggaaaaga + 4921 aaagaggcgt actgcctgaa cgagaagcta tcaccgccca gcctaaacgg atatcatcat + 4981 cgctcatcca tgtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag + 5041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc + 5101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag + 5161 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac + 5221 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggagctg + 5281 taatataaaa accttcttca actaacgggg caggttagtg acattagaaa accgactgta + 5341 aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat + 5401 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc + 5461 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata + 5521 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc + 5581 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc + 5641 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca + 5701 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa + 5761 agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa + 5821 gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat + 5881 ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa + 5941 gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca + 6001 attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg + 6061 cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa + 6121 cataaatata tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg + 6181 gtgctttagt tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa + 6241 ggatttgagc gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg + 6301 ccgatgataa gctgtcaaac atgagaattc gcggccgcgc tagcaaagga gaagaacttt + 6361 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt + 6421 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt + 6481 gcactactgg aaaactacct gttccatggc caacacttgt cactactttg acctatggtg + 6541 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca + 6601 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga + 6661 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta + 6721 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac + 6781 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc + 6841 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa + 6901 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt + 6961 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg + 7021 ggattacaca tggcatggat gagctctaca aataataagg ccggccaata aagatcctag + 7081 gacgccgcca agccagctta aacccagctc aatgagctgg gttttttgtt tgttaaaaat + 7141 gaagaagaaa ctgtgaagcg tatttaacta gtaaaatcta agacatatca tgatttataa + 7201 aaacaaaaaa accttgcagg atatgcaagg cttctggatg acccgtacgg gattcgaact + 7261 ccggatgtga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa + 7321 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatcgatca + 7381 gaccagtttt taatttgtgt gtttccatgt gtccagtttg gaatactctt aacctcattg + 7441 gaaatcgcgg cataatcact ggtggtatga ttgatgaccg cgtcaacaat gacctttatg + 7501 ccatattctt cagcggctgc acacatttct ttaaattctt gttcagtacc taagtaacgg + 7561 ttgccaattt gatacgatgt cggctgatac agccagtacc agttcgacat gcttttatct + 7621 ccttgattcc cttcctttac ttggttaatc ggagatgtct gaatggctgt atatcctgca + 7681 tcatgaatat ccttcatatt gtgttttaac gtattgaacg accaattcca tgcatgaaga + 7741 atggttccgc ttttgatcga cggtgctgta agctcattcg atttgttcgc cgtttcagca + 7801 ctcgcagccg ccggtcctgc cagaaccaaa tgaaacagca ataaaaatcc agcgaataac + 7861 ggcagtaaag aggttttgaa tcgttttgca aacattcttg acactcctta tttgattttt + 7921 tgaagactta cttcggagtc aaaaatccct cttacttcat tcttccgctt cctcctttca + 7981 aaccgatgtg aagactggag aattttgtt +// \ No newline at end of file