# HG changeset patch # User Jonathan Tellechea # Date 2019-12-11 16:01:21 # Node ID 84fd403c44e61624034f18c34c3ec63897943a5f # Parent 9117870bc39bc4e321a6efd937ab9942a8170364 Vector used to barcode B.subtilis using CRISPR diff --git a/pBS-CRISPR-Vector.gb b/pBS-CRISPR-Vector.gb new file mode 100644 --- /dev/null +++ b/pBS-CRISPR-Vector.gb @@ -0,0 +1,193 @@ +LOCUS pBS-CRISPR-Vector 8410 bp ds-DNA circular 11-DEC-2019 +DEFINITION . +ACCESSION pJOE8999.1 +SOURCE Unknown. + ORGANISM Unknown Unclassified. +FEATURES Location/Qualifiers + CDS complement(4120..8222) + /label="cas9 CDS" + /ApEinfo_revcolor=#b4abac + /ApEinfo_fwdcolor=#b4abac + misc_feature complement(58..731) + /label="pUCori misc_feature" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + CDS complement(831..1601) + /label="kanR CDS" + /ApEinfo_revcolor=#c6c9d1 + /ApEinfo_fwdcolor=#c6c9d1 + CDS complement(8252..8361) + /label="Pman CDS" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + misc_feature complement(1716..2830) + /label="rep pE194ts misc_feature" + /ApEinfo_revcolor=#84b0dc + /ApEinfo_fwdcolor=#84b0dc + CDS 3021..3050 + /label="oop ter CDS" + /ApEinfo_revcolor=#d59687 + /ApEinfo_fwdcolor=#d59687 + CDS 2891..2971 + /label="'gRNA CDS" + /ApEinfo_revcolor=#85dae9 + /ApEinfo_fwdcolor=#85dae9 + misc_feature 3059..3084 + /label="T7P misc_feature" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + misc_feature 2842..2851 + /label="PvanP* misc_feature" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 + misc_feature 3085..3569 + /label="left_homology" + /ApEinfo_revcolor=#9eafd2 + /ApEinfo_fwdcolor=#9eafd2 + misc_feature 3579..4075 + /label="right_homology" + /ApEinfo_revcolor=#c6c9d1 + /ApEinfo_fwdcolor=#c6c9d1 +ORIGIN + 1 gaatggccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg + 61 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc + 121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc + 181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt + 241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc + 301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact + 361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac + 421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag + 481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg + 541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg + 601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga + 661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt + 721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct + 781 ttgagtgagc tgaaattatg aggggatctc tcagagctcg aggtcatcgt tcaaaatggt + 841 atgcgttttg acacatccac tatatatccg tgtcgttctg tccactcctg aatcccattc + 901 cagaaattct ctagcgattc cagaagtttc tcagagtcgg aaagttgacc agacattacg + 961 aactggcaca gatggtcata acctgaagga agatctgatt gcttaactgc ttcagttaag + 1021 accgaagcgc tcgtcgtata acagatgcga tgatgcagac caatcaacat ggcacctgcc + 1081 attgctacct gcacagtcaa ggatggtaga aatgttgtcg gtccttgcac acgaatatta + 1141 cgccatttgc ctgcatattc aaacagctct tctacgataa gggcacaaat cgcatcgtgg + 1201 aacgtttggg cttctaccga tttagcagtt tgatacactt tctctaagta tccacctgaa + 1261 tcataaatcg gcaaaataga gaaaaattga ccatgtgtaa gcggccaatc tgattccacc + 1321 tgagatgcat aatctagtag aatctcttcg ctatcaaaat tcacttccac cttccactca + 1381 ccggttgtcc attcatggct gaactctgct tcctctgttg acatgacaca catcatctca + 1441 atatccgaat agggcccatc agtctgacga ccaagagagc cataaacacc aatagcctta + 1501 acatcatccc catatttatc caatattcgt tccttaattt catgaacaat cttcattctt + 1561 tcttctctag tcattattat tggtccattc actattctca ttcccttttc agataatttt + 1621 agatttgctt ttctaaataa gaatatttgg agagcaccgt tcttattcag ctattaaacc + 1681 cattatatcg ggtttttgag gggatttcaa ctgcagacac ctaaattcaa aatctatcgg + 1741 tcagatttat accgatttga ttttatatat tcttgaataa catacgccga gttatcacat + 1801 aaaagcggga accaatcatc aaatttaaac ttcattgcat aatccattaa actcttaaat + 1861 tctacgattc cttgttcatc aataaactca atcatttctt taattaattt atatctatct + 1921 gttgttgttt tctttaataa ttcatcaaca tctacaccgc cataaactat catatcttct + 1981 ttttgatatt taaatttatt aggatcgtcc atgtgaagca tatatctcac aagacctttc + 2041 acacttcctg caatctgcgg aatagtcgca ttcaattctt ctgttaatta tttttatctg + 2101 ttcataagat ttattaccct catacatcac tagaatatga taatgctctt ttttcatcct + 2161 accttctgta tcagtatccc tatcatgtaa tggagacact acaaattgaa tgtgtaactc + 2221 ttttaaatac tctaaccact cggcttttgc tgattctgga tataaaacaa atgtccaatt + 2281 acgtcctctt gaatttttct tgttttcagt ttcttttatt acattttcgc tcatgatata + 2341 ataacggtgc taatacattt aacaaaattt agtcatagat aggcagcatg ccagtgctgt + 2401 ctatcttttt ttgtttaaaa tgcaccgtat tcctcctttg catatttttt tattagaata + 2461 ccggttgcat ctgatttgct aatattatat ttttctttga ttctatttaa tatctcattt + 2521 tcttctgttg taagtcttaa agtaacagca acttttttct cttcttttct atctacaacc + 2581 atcactgtac ctcccaacat ctgttttttt cactttaaca taaaaaacaa ccttttaaca + 2641 ttaaaaaccc aatatttatt tatttgtttg gacaatggac aatggacacc taggggggag + 2701 gtcgtagtac ccccctatgt tttctcccct aaataacccc aaaaatctaa gaaaaaaaga + 2761 cctcaaaaag gtctttaatt aacatctcaa atttcgcatt tattccaatt tcctttttgc + 2821 gtgtgatgcg aattcttgac cgtgattaga gaattgagta aaatgtacct ATTTAGAGCC + 2881 CTGCCGTGCA gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac + 2941 ttgaaaaagt ggcaccgagt cggtgctttt tactccatct ggatttgttc agaacgctcg + 3001 gttgccgccg ggcgtttttt atctaaagct taggcccagt cgaaagactg ggccttttta + 3061 atacgactca ctatagggtc gacgagcaga aggaaaggtg ttcataaaat ggatgttaca + 3121 ctcggttatt tgcgcgaatc actttcgaat catctagaaa atgaagtctg ccagcgtatt + 3181 tgcaagaaga tgctggcgaa gcgttatgca aatgaagagg aatttgtaaa agacttagat + 3241 gataatgaaa tgtctttttt gaaccatgta ttagaaaaag aaattaagta tgcccagaat + 3301 gaacaggatc aaaaacgggc caaggaatta aacgaggtgt atgaattact gctttgagaa + 3361 aggcgggtaa gaaatgggag caattgaacg aagcggatat acatttcagc cggagtttag + 3421 cgttgtccgt caaaacggag ccatccatgt gtaccatcaa ggagaatttg tggaagaaat + 3481 cgaatttgaa tttaacgggg aatatcccga tcatgatttg attgaagagc ttgtgaatca + 3541 ttattgtttt gagcatgaga tttagagccA CTAGTtaaat ttaggatgta atcaggccat + 3601 actttttcag gatatccacg gtgatgtttt tggcttttgt gccggaatca tcgacattgg + 3661 tgacaaagac ataagagcca tgctctgttt tgatgaatcc gacaaaccat cctaatccca + 3721 tatcagtcag gcgggttccg gttttcccgt agagcgtgta atgatcgcct tcctcttgaa + 3781 tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa tggaagctcc tctttagcca + 3841 gtttctccaa aaaagtttct tgttccagcg gagatattgt gagcgagctt tgcagccaaa + 3901 actggtcgat tcccccgctg atatcctcgt tgccgtaaga tagagtatga agccatgttt + 3961 tcattctttc ctcgccgata tctctcgcta atgcctgata ataccagatt gcggattcgc + 4021 gcatggccga gccaagcgta tggtcgcggt tccatgattc gaattcacgc ttgacccttt + 4081 ctagattaag aaataatctt catctaaaat atacttcagt cacctcctag ctgactcaaa + 4141 tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact + 4201 tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg + 4261 ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt + 4321 ggtttgtctc tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa + 4381 ataacacgct tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc + 4441 tgctccacaa acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa + 4501 tgactagcta aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt + 4561 tgtaattctc cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta + 4621 tatttaggta gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa + 4681 aagtcaatcg gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct + 4741 ttaacggatt ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa + 4801 taagctaccg ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga + 4861 gcaataagct tgtccgaatt tctttttggt aaaattgact ccttggagaa tccgcctgtc + 4921 tgtacttctg ttttcttgac aatattgact tggggcatgg acaatacttt gcgcactgtg + 4981 gcaaaatctc gccctttatc ccagacaatt tctccagttt ccccattagt ttcgattaga + 5041 gggcgtttgc gaatctctcc atttgcaagt gtaatttctg ttttgaagaa gttcatgata + 5101 ttagagtaaa agaaatattt tgcggttgct ttgcctattt cttgctcaga cttagcaatc + 5161 attttacgaa catcataaac tttataatca ccatagacaa actccgattc aagttttgga + 5221 tatttcttaa tcaaagcagt tccaacgacg gcatttagat acgcatcatg ggcatgatgg + 5281 taattgttaa tctcacgtac tttatagaat tggaaatctt ttcggaagtc agaaactaat + 5341 ttagatttta aggtaatcac tttaacctct cgaataagtt tatcattttc atcgtattta + 5401 gtattcatgc gactatccaa aatttgtgcc acatgcttag tgatttggcg agtttcaacc + 5461 aattggcgtt tgataaaacc agctttatca agttcactca aacctccacg ttcagctttc + 5521 gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg tctccaatag + 5581 tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc acgattttta + 5641 tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact ttgtggaaca + 5701 atgtgatcga catcataatc acttaaacga ttaatatcta attcttggtc cacatacatg + 5761 tctcttccat tttggagata atagagatag agcttttcat tttgcaattg agtattttca + 5821 acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc gattcgtttc + 5881 atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg tgccatttca + 5941 ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc aacaactttt + 6001 acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc aatatgttca + 6061 tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt aaatgtcaaa + 6121 ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga tttcaaaaaa + 6181 tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt tcgagacaaa + 6241 cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc atcaaagagg + 6301 tgagcatatg ttttaagtct ttcctcaatc atctccctat cttcaaataa ggtcaatgtt + 6361 aaaacaatat cctctaagat atcttcattt tcttcattat ccaaaaaatc tttatcttta + 6421 ataattttta gcaaatcatg gtaggtacct aatgaagcat taaatctatc ttcaactcct + 6481 gaaatttcaa cactatcaaa acattctatt tttttgaaat aatcttcttt taattgctta + 6541 acggttactt ttcgatttgt tttgaagagt aaatcaacaa tggctttctt ctgttcacct + 6601 gaaagaaatg ctggttttcg cattccttca gtaacatatt tgacctttgt caattcgtta + 6661 taaaccgtaa aatactcata aagcaaacta tgttttggta gtactttttc atttggaaga + 6721 tttttatcaa agtttgtcat gcgttcaata aatgattgag ctgaagcacc tttatcgaca + 6781 acttcttcaa aattccatgg ggtaattgtt tcttcagact tccgagtcat ccatgcaaaa + 6841 cgactattgc cacgcgccaa tggaccaaca taataaggaa ttcgaaaagt caagattttt + 6901 tcaatcttct cacgattgtc ttttaaaaat ggataaaagt cttcttgtct tctcaaaata + 6961 gcatgcagct cacccaagtg aatttgatgg ggaatagagc cgttgtcaaa ggtccgttgc + 7021 ttgcgcagca aatcttcacg atttagtttc accaataatt cctcagtacc atccattttt + 7081 tctaaaattg gtttgataaa tttataaaat tcttcttggc tagctccccc atcaatataa + 7141 cctgcatatc cgttttttga ttgatcaaaa aagatttctt tatacttttc tggaagttgt + 7201 tgtcgaacta aagcttttaa aagagtcaag tcttgatgat gttcatcgta gcgtttaatc + 7261 attgaagctg ataggggagc cttagttatt tcagtattta ctcttaggat atctgaaagt + 7321 aaaatagcat ctgataaatt cttagctgcc aaaaacaaat cagcatattg atctccaatt + 7381 tgcgccaata aattatctaa atcatcatcg taagtatctt ttgaaagctg taatttagca + 7441 tcttctgcca aatcaaaatt tgatttaaaa ttaggggtca aacccaatga caaagcaatg + 7501 agattcccaa ataagccatt tttcttctca ccggggagct gagcaatgag attttctaat + 7561 cgtcttgatt tactcaatcg tgcagaaaga atcgctttag catctactcc acttgcgtta + 7621 atagggtttt cttcaaataa ttgattgtag gtttgtacca actggataaa tagtttgtcc + 7681 acatcactat tatcaggatt taaatctccc tcaatcaaaa aatgaccacg aaacttaatc + 7741 atatgcgcta aggccaaata gattaagcgc aaatccgctt tatcagtaga atctaccaat + 7801 ttttttcgca gatgatagat agttggatat ttctcatgat aagcaacttc atctactata + 7861 tttccaaaaa taggatgacg ttcatgcttc ttgtcttctt ccaccaaaaa agactcttca + 7921 agtcgatgaa agaaactatc atctactttc gccatctcat ttgaaaaaat ctcctgtaga + 7981 taacaaatac gattcttccg acgtgtatac cttctacgag ctgtccgttt gagacgagtc + 8041 gcttccgctg tctctccact gtcaaataaa agagccccta taagattttt tttgatactg + 8101 tggcggtctg tatttcccag aaccttgaac tttttagacg gaaccttata atcatcagtg + 8161 atcaccgccc atccgacgct atttgtgccg atatctaagc ctattgagta tttcttatcc + 8221 atttttgcct cctaacttaa gaataagatc ttgtctcaac tgtataccga aatcagctca + 8281 ttaaaatcgc tttttttacc ataggttccg gtaataaagg catttttccc tataacaaaa + 8341 aaagcaagga ataatccctg cttttaataa tccaaatgag ataaaaatgt catgacattg + 8401 gtgtacagaa +// \ No newline at end of file