# HG changeset patch # User Jonathan Tellechea # Date 2019-12-11 15:59:44 # Node ID 9117870bc39bc4e321a6efd937ab9942a8170364 # Parent acc4afc323497db3f52cd9edba5a19f2d76f97f2 Vector used to barcode B.subtilis using CreLox diff --git a/pBS-CreLox-Vector.gb b/pBS-CreLox-Vector.gb new file mode 100644 --- /dev/null +++ b/pBS-CreLox-Vector.gb @@ -0,0 +1,127 @@ +LOCUS pBS-CreLox-Vector 4456 bp ds-DNA circular 11-DEC-2019 +DEFINITION . +ACCESSION pTarget +SOURCE Unknown. + ORGANISM Unknown Unclassified. +FEATURES Location/Qualifiers + rep_origin join(4455..4456,1..681) + /label="ColE1 origin" + /ApEinfo_revcolor=#9eafd2 + /ApEinfo_fwdcolor=#9eafd2 + misc_feature 2399..2895 + /label="Right Homology" + /ApEinfo_revcolor=#d59687 + /ApEinfo_fwdcolor=#d59687 + misc_feature 2399..3398 + /label="Right Homology" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + misc_feature 1887..1920 + /label="lox71" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 + misc_feature 1396..1880 + /label="Left Homology" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + misc_feature 2365..2398 + /label="lox66" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + misc_feature 883..1880 + /label="Left homology" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + misc_feature 1887..2398 + /label="lox71-zeocin-lox66" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + CDS 3565..4357 + /label="aadA_spec/strep_resistance" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + CDS 75..663 + /label="pMB1" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + misc_feature complement(3583..3603) + /label="SEQ and PCR" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 +ORIGIN + 1 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag + 61 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa + 121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg + 181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag + 241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg + 301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga + 361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc + 421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc + 481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga + 541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt + 601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg + 661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac + 721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga + 781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg + 841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcaagaagat ttcacgcaat + 901 ggtgtgccgc tgtatggcac aattgcggtg ctgatcggtt tggctgtcgg cgttgtactg + 961 aactatatcg cgccgccgaa gatctttgtc tatgtataca gcgcaagcgt gctcccaggg + 1021 atgattcctt ggtttatcat tttgatcagc catatcggat tcagaaaagc gaagggtgct + 1081 gcattagata aacatccatt caaaatgccg tttgctcctt ttacaaacta tttgacgatt + 1141 gctttcttgc tgatggttct ggttggcatg tggtttaacg atgatactcg tatctccctg + 1201 attgtaggcg tgattttctt agcgcttgtg gtcattagtt attatgtgtt tgggattggc + 1261 aaacgcacgc aggccaattt gacaaaaagc gaacaagcag ctgaataacg ataaaaaaga + 1321 gacattcacg gatgtctctt tttttatttt tcgcgtatgg attttcccta ttttagcaag + 1381 ctatagggta aagatagcag aaggaaaggt gttcataaaa tggatgttac actcggttat + 1441 ttgcgcgaat cactttcgaa tcatctagaa aatgaagtct gccagcgtat ttgcaagaag + 1501 atgctggcga agcgttatgc aaatgaagag gaatttgtaa aagacttaga tgataatgaa + 1561 atgtcttttt tgaaccatgt attagaaaaa gaaattaagt atgcccagaa tgaacaggat + 1621 caaaaacggg ccaaggaatt aaacgaggtg tatgaattac tgctttgaga aaggcgggta + 1681 agaaatggga gcaattgaac gaagcggata tacatttcag ccggagttta gcgttgtccg + 1741 tcaaaacgga gccatccatg tgtaccatca aggagaattt gtggaagaaa tcgaatttga + 1801 atttaacggg gaatatcccg atcatgattt gattgaagag cttgtgaatc attattgttt + 1861 tgagcatgag atttagagcc ACTAGTtacc gttcgtatag catacattat acgaagttat + 1921 gtttttcatg aaagacttga tatggctttt tatatgtgtt actctacata cagaaaggag + 1981 gaactaaata tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc + 2041 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac + 2101 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg + 2161 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag + 2221 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc + 2281 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac + 2341 ttcgtggccg aggagcagga ctgaataact tcgtatagca tacattatac gaacggtaat + 2401 ttaggatgta atcaggccat actttttcag gatatccacg gtgatgtttt tggcttttgt + 2461 gccggaatca tcgacattgg tgacaaagac ataagagcca tgctctgttt tgatgaatcc + 2521 gacaaaccat cctaatccca tatcagtcag gcgggttccg gttttcccgt agagcgtgta + 2581 atgatcgcct tcctcttgaa tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa + 2641 tggaagctcc tctttagcca gtttctccaa aaaagtttct tgttccagcg gagatattgt + 2701 gagcgagctt tgcagccaaa actggtcgat tcccccgctg atatcctcgt tgccgtaaga + 2761 tagagtatga agccatgttt tcattctttc ctcgccgata tctctcgcta atgcctgata + 2821 ataccagatt gcggattcgc gcatggccga gccaagcgta tggtcgcggt tccatgattc + 2881 gaattcacgc ttgaccccgt cccatcgttt aacatcgtat tcatctctta cagctttgac + 2941 ctgcaaaccg attaaagcgt tcacgacttt aaaggtggat tgcggagttt gtctttgatt + 3001 tgcccgtttt ctgttataga caaatgtctg atccttttgt aaatcgtgaa gaatgaaggt + 3061 tccatccgta tccttgaatt catcatcgac attcattttg ctgacattca agtgtttttc + 3121 atgagcagat gatgctgcgt ggacggagaa gccgccgatc cctgcaatac tcagcacaag + 3181 cacaacatat atccattttt tcattttgtt ctccccttcg ttttgattaa ctaccttttc + 3241 tctctttcgt ttgttcttcc tttaaaaaat aatcaaaaag cgcttaaatt tggttttgca + 3301 aacaaatact gttactgggt ttatagtcgg aagataacgt gaaaatagag ggtaaaaagg + 3361 aaggcagggt aagcggatga atgagcagat tccacatggt cgacctgcag aagcttagat + 3421 ctattaccct gttatcccta ctcgagttca tgtgcagctc catcagcaaa aggggatgat + 3481 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg + 3541 tcatcagcgg tggagtgcaa tgtcatgagg gaagcggtga tcgccgaagt atcgactcaa + 3601 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat + 3661 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt + 3721 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa + 3781 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg + 3841 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg + 3901 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct + 3961 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa + 4021 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta + 4081 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc + 4141 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca + 4201 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt + 4261 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac + 4321 gtgaaaggcg agatcaccaa ggtagtcggc aaataagatg ccgctcgcca gtcgattggc + 4381 tgagctcatg aagttcctat tccgaagttc cgcgaacgcg taaaggatct aggtgaagat + 4441 cctttttgat aatctc +// \ No newline at end of file