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j.tellechea-luzardo@newcastle.ac.uk
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| 1 |
LOCUS pBS-CRISPR-Vector 8410 bp ds-DNA circular 11-DEC-2019 |
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| 2 |
DEFINITION . |
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| 3 |
ACCESSION pJOE8999.1 |
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| 4 |
SOURCE Unknown. |
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| 5 |
ORGANISM Unknown Unclassified. |
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| 6 |
FEATURES Location/Qualifiers |
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| 7 |
CDS complement(4120..8222) |
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| 8 |
/label="cas9 CDS" |
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| 9 |
/ApEinfo_revcolor=#b4abac |
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| 10 |
/ApEinfo_fwdcolor=#b4abac |
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| 11 |
misc_feature complement(58..731) |
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| 12 |
/label="pUCori misc_feature" |
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| 13 |
/ApEinfo_revcolor=#c7b0e3 |
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| 14 |
/ApEinfo_fwdcolor=#c7b0e3 |
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| 15 |
CDS complement(831..1601) |
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| 16 |
/label="kanR CDS" |
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| 17 |
/ApEinfo_revcolor=#c6c9d1 |
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| 18 |
/ApEinfo_fwdcolor=#c6c9d1 |
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| 19 |
CDS complement(8252..8361) |
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| 20 |
/label="Pman CDS" |
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| 21 |
/ApEinfo_revcolor=#b7e6d7 |
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| 22 |
/ApEinfo_fwdcolor=#b7e6d7 |
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| 23 |
misc_feature complement(1716..2830) |
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| 24 |
/label="rep pE194ts misc_feature" |
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| 25 |
/ApEinfo_revcolor=#84b0dc |
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| 26 |
/ApEinfo_fwdcolor=#84b0dc |
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| 27 |
CDS 3021..3050 |
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| 28 |
/label="oop ter CDS" |
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| 29 |
/ApEinfo_revcolor=#d59687 |
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| 30 |
/ApEinfo_fwdcolor=#d59687 |
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| 31 |
CDS 2891..2971 |
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| 32 |
/label="'gRNA CDS" |
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| 33 |
/ApEinfo_revcolor=#85dae9 |
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| 34 |
/ApEinfo_fwdcolor=#85dae9 |
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| 35 |
misc_feature 3059..3084 |
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| 36 |
/label="T7P misc_feature" |
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| 37 |
/ApEinfo_revcolor=#75c6a9 |
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| 38 |
/ApEinfo_fwdcolor=#75c6a9 |
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| 39 |
misc_feature 2842..2851 |
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| 40 |
/label="PvanP* misc_feature" |
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| 41 |
/ApEinfo_revcolor=#faac61 |
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| 42 |
/ApEinfo_fwdcolor=#faac61 |
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| 43 |
misc_feature 3085..3569 |
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| 44 |
/label="left_homology" |
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| 45 |
/ApEinfo_revcolor=#9eafd2 |
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| 46 |
/ApEinfo_fwdcolor=#9eafd2 |
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| 47 |
misc_feature 3579..4075 |
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| 48 |
/label="right_homology" |
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| 49 |
/ApEinfo_revcolor=#c6c9d1 |
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| 50 |
/ApEinfo_fwdcolor=#c6c9d1 |
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| 51 |
ORIGIN |
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| 52 |
1 gaatggccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg |
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| 53 |
61 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc |
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| 54 |
121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc |
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| 55 |
181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt |
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| 56 |
241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc |
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| 57 |
301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact |
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| 58 |
361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac |
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| 59 |
421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag |
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| 60 |
481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg |
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| 61 |
541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg |
|
| 62 |
601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga |
|
| 63 |
661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt |
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| 64 |
721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct |
|
| 65 |
781 ttgagtgagc tgaaattatg aggggatctc tcagagctcg aggtcatcgt tcaaaatggt |
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| 66 |
841 atgcgttttg acacatccac tatatatccg tgtcgttctg tccactcctg aatcccattc |
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| 67 |
901 cagaaattct ctagcgattc cagaagtttc tcagagtcgg aaagttgacc agacattacg |
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| 68 |
961 aactggcaca gatggtcata acctgaagga agatctgatt gcttaactgc ttcagttaag |
|
| 69 |
1021 accgaagcgc tcgtcgtata acagatgcga tgatgcagac caatcaacat ggcacctgcc |
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| 70 |
1081 attgctacct gcacagtcaa ggatggtaga aatgttgtcg gtccttgcac acgaatatta |
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| 71 |
1141 cgccatttgc ctgcatattc aaacagctct tctacgataa gggcacaaat cgcatcgtgg |
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| 72 |
1201 aacgtttggg cttctaccga tttagcagtt tgatacactt tctctaagta tccacctgaa |
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| 73 |
1261 tcataaatcg gcaaaataga gaaaaattga ccatgtgtaa gcggccaatc tgattccacc |
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| 74 |
1321 tgagatgcat aatctagtag aatctcttcg ctatcaaaat tcacttccac cttccactca |
|
| 75 |
1381 ccggttgtcc attcatggct gaactctgct tcctctgttg acatgacaca catcatctca |
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| 76 |
1441 atatccgaat agggcccatc agtctgacga ccaagagagc cataaacacc aatagcctta |
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| 77 |
1501 acatcatccc catatttatc caatattcgt tccttaattt catgaacaat cttcattctt |
|
| 78 |
1561 tcttctctag tcattattat tggtccattc actattctca ttcccttttc agataatttt |
|
| 79 |
1621 agatttgctt ttctaaataa gaatatttgg agagcaccgt tcttattcag ctattaaacc |
|
| 80 |
1681 cattatatcg ggtttttgag gggatttcaa ctgcagacac ctaaattcaa aatctatcgg |
|
| 81 |
1741 tcagatttat accgatttga ttttatatat tcttgaataa catacgccga gttatcacat |
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| 82 |
1801 aaaagcggga accaatcatc aaatttaaac ttcattgcat aatccattaa actcttaaat |
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| 83 |
1861 tctacgattc cttgttcatc aataaactca atcatttctt taattaattt atatctatct |
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| 84 |
1921 gttgttgttt tctttaataa ttcatcaaca tctacaccgc cataaactat catatcttct |
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| 85 |
1981 ttttgatatt taaatttatt aggatcgtcc atgtgaagca tatatctcac aagacctttc |
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| 86 |
2041 acacttcctg caatctgcgg aatagtcgca ttcaattctt ctgttaatta tttttatctg |
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| 87 |
2101 ttcataagat ttattaccct catacatcac tagaatatga taatgctctt ttttcatcct |
|
| 88 |
2161 accttctgta tcagtatccc tatcatgtaa tggagacact acaaattgaa tgtgtaactc |
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| 89 |
2221 ttttaaatac tctaaccact cggcttttgc tgattctgga tataaaacaa atgtccaatt |
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| 90 |
2281 acgtcctctt gaatttttct tgttttcagt ttcttttatt acattttcgc tcatgatata |
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| 91 |
2341 ataacggtgc taatacattt aacaaaattt agtcatagat aggcagcatg ccagtgctgt |
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| 92 |
2401 ctatcttttt ttgtttaaaa tgcaccgtat tcctcctttg catatttttt tattagaata |
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| 93 |
2461 ccggttgcat ctgatttgct aatattatat ttttctttga ttctatttaa tatctcattt |
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| 94 |
2521 tcttctgttg taagtcttaa agtaacagca acttttttct cttcttttct atctacaacc |
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| 95 |
2581 atcactgtac ctcccaacat ctgttttttt cactttaaca taaaaaacaa ccttttaaca |
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| 96 |
2641 ttaaaaaccc aatatttatt tatttgtttg gacaatggac aatggacacc taggggggag |
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| 97 |
2701 gtcgtagtac ccccctatgt tttctcccct aaataacccc aaaaatctaa gaaaaaaaga |
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| 98 |
2761 cctcaaaaag gtctttaatt aacatctcaa atttcgcatt tattccaatt tcctttttgc |
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| 99 |
2821 gtgtgatgcg aattcttgac cgtgattaga gaattgagta aaatgtacct ATTTAGAGCC |
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| 100 |
2881 CTGCCGTGCA gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac |
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| 101 |
2941 ttgaaaaagt ggcaccgagt cggtgctttt tactccatct ggatttgttc agaacgctcg |
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| 102 |
3001 gttgccgccg ggcgtttttt atctaaagct taggcccagt cgaaagactg ggccttttta |
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| 103 |
3061 atacgactca ctatagggtc gacgagcaga aggaaaggtg ttcataaaat ggatgttaca |
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| 104 |
3121 ctcggttatt tgcgcgaatc actttcgaat catctagaaa atgaagtctg ccagcgtatt |
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| 105 |
3181 tgcaagaaga tgctggcgaa gcgttatgca aatgaagagg aatttgtaaa agacttagat |
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| 106 |
3241 gataatgaaa tgtctttttt gaaccatgta ttagaaaaag aaattaagta tgcccagaat |
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| 107 |
3301 gaacaggatc aaaaacgggc caaggaatta aacgaggtgt atgaattact gctttgagaa |
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| 108 |
3361 aggcgggtaa gaaatgggag caattgaacg aagcggatat acatttcagc cggagtttag |
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| 109 |
3421 cgttgtccgt caaaacggag ccatccatgt gtaccatcaa ggagaatttg tggaagaaat |
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| 110 |
3481 cgaatttgaa tttaacgggg aatatcccga tcatgatttg attgaagagc ttgtgaatca |
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| 111 |
3541 ttattgtttt gagcatgaga tttagagccA CTAGTtaaat ttaggatgta atcaggccat |
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| 112 |
3601 actttttcag gatatccacg gtgatgtttt tggcttttgt gccggaatca tcgacattgg |
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| 113 |
3661 tgacaaagac ataagagcca tgctctgttt tgatgaatcc gacaaaccat cctaatccca |
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| 114 |
3721 tatcagtcag gcgggttccg gttttcccgt agagcgtgta atgatcgcct tcctcttgaa |
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| 115 |
3781 tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa tggaagctcc tctttagcca |
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| 116 |
3841 gtttctccaa aaaagtttct tgttccagcg gagatattgt gagcgagctt tgcagccaaa |
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| 117 |
3901 actggtcgat tcccccgctg atatcctcgt tgccgtaaga tagagtatga agccatgttt |
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| 118 |
3961 tcattctttc ctcgccgata tctctcgcta atgcctgata ataccagatt gcggattcgc |
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| 119 |
4021 gcatggccga gccaagcgta tggtcgcggt tccatgattc gaattcacgc ttgacccttt |
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| 120 |
4081 ctagattaag aaataatctt catctaaaat atacttcagt cacctcctag ctgactcaaa |
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| 121 |
4141 tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact |
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| 122 |
4201 tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg |
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| 123 |
4261 ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt |
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| 124 |
4321 ggtttgtctc tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa |
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| 125 |
4381 ataacacgct tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc |
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| 126 |
4441 tgctccacaa acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa |
|
| 127 |
4501 tgactagcta aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt |
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| 128 |
4561 tgtaattctc cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta |
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| 129 |
4621 tatttaggta gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa |
|
| 130 |
4681 aagtcaatcg gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct |
|
| 131 |
4741 ttaacggatt ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa |
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| 132 |
4801 taagctaccg ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga |
|
| 133 |
4861 gcaataagct tgtccgaatt tctttttggt aaaattgact ccttggagaa tccgcctgtc |
|
| 134 |
4921 tgtacttctg ttttcttgac aatattgact tggggcatgg acaatacttt gcgcactgtg |
|
| 135 |
4981 gcaaaatctc gccctttatc ccagacaatt tctccagttt ccccattagt ttcgattaga |
|
| 136 |
5041 gggcgtttgc gaatctctcc atttgcaagt gtaatttctg ttttgaagaa gttcatgata |
|
| 137 |
5101 ttagagtaaa agaaatattt tgcggttgct ttgcctattt cttgctcaga cttagcaatc |
|
| 138 |
5161 attttacgaa catcataaac tttataatca ccatagacaa actccgattc aagttttgga |
|
| 139 |
5221 tatttcttaa tcaaagcagt tccaacgacg gcatttagat acgcatcatg ggcatgatgg |
|
| 140 |
5281 taattgttaa tctcacgtac tttatagaat tggaaatctt ttcggaagtc agaaactaat |
|
| 141 |
5341 ttagatttta aggtaatcac tttaacctct cgaataagtt tatcattttc atcgtattta |
|
| 142 |
5401 gtattcatgc gactatccaa aatttgtgcc acatgcttag tgatttggcg agtttcaacc |
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| 143 |
5461 aattggcgtt tgataaaacc agctttatca agttcactca aacctccacg ttcagctttc |
|
| 144 |
5521 gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg tctccaatag |
|
| 145 |
5581 tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc acgattttta |
|
| 146 |
5641 tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact ttgtggaaca |
|
| 147 |
5701 atgtgatcga catcataatc acttaaacga ttaatatcta attcttggtc cacatacatg |
|
| 148 |
5761 tctcttccat tttggagata atagagatag agcttttcat tttgcaattg agtattttca |
|
| 149 |
5821 acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc gattcgtttc |
|
| 150 |
5881 atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg tgccatttca |
|
| 151 |
5941 ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc aacaactttt |
|
| 152 |
6001 acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc aatatgttca |
|
| 153 |
6061 tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt aaatgtcaaa |
|
| 154 |
6121 ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga tttcaaaaaa |
|
| 155 |
6181 tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt tcgagacaaa |
|
| 156 |
6241 cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc atcaaagagg |
|
| 157 |
6301 tgagcatatg ttttaagtct ttcctcaatc atctccctat cttcaaataa ggtcaatgtt |
|
| 158 |
6361 aaaacaatat cctctaagat atcttcattt tcttcattat ccaaaaaatc tttatcttta |
|
| 159 |
6421 ataattttta gcaaatcatg gtaggtacct aatgaagcat taaatctatc ttcaactcct |
|
| 160 |
6481 gaaatttcaa cactatcaaa acattctatt tttttgaaat aatcttcttt taattgctta |
|
| 161 |
6541 acggttactt ttcgatttgt tttgaagagt aaatcaacaa tggctttctt ctgttcacct |
|
| 162 |
6601 gaaagaaatg ctggttttcg cattccttca gtaacatatt tgacctttgt caattcgtta |
|
| 163 |
6661 taaaccgtaa aatactcata aagcaaacta tgttttggta gtactttttc atttggaaga |
|
| 164 |
6721 tttttatcaa agtttgtcat gcgttcaata aatgattgag ctgaagcacc tttatcgaca |
|
| 165 |
6781 acttcttcaa aattccatgg ggtaattgtt tcttcagact tccgagtcat ccatgcaaaa |
|
| 166 |
6841 cgactattgc cacgcgccaa tggaccaaca taataaggaa ttcgaaaagt caagattttt |
|
| 167 |
6901 tcaatcttct cacgattgtc ttttaaaaat ggataaaagt cttcttgtct tctcaaaata |
|
| 168 |
6961 gcatgcagct cacccaagtg aatttgatgg ggaatagagc cgttgtcaaa ggtccgttgc |
|
| 169 |
7021 ttgcgcagca aatcttcacg atttagtttc accaataatt cctcagtacc atccattttt |
|
| 170 |
7081 tctaaaattg gtttgataaa tttataaaat tcttcttggc tagctccccc atcaatataa |
|
| 171 |
7141 cctgcatatc cgttttttga ttgatcaaaa aagatttctt tatacttttc tggaagttgt |
|
| 172 |
7201 tgtcgaacta aagcttttaa aagagtcaag tcttgatgat gttcatcgta gcgtttaatc |
|
| 173 |
7261 attgaagctg ataggggagc cttagttatt tcagtattta ctcttaggat atctgaaagt |
|
| 174 |
7321 aaaatagcat ctgataaatt cttagctgcc aaaaacaaat cagcatattg atctccaatt |
|
| 175 |
7381 tgcgccaata aattatctaa atcatcatcg taagtatctt ttgaaagctg taatttagca |
|
| 176 |
7441 tcttctgcca aatcaaaatt tgatttaaaa ttaggggtca aacccaatga caaagcaatg |
|
| 177 |
7501 agattcccaa ataagccatt tttcttctca ccggggagct gagcaatgag attttctaat |
|
| 178 |
7561 cgtcttgatt tactcaatcg tgcagaaaga atcgctttag catctactcc acttgcgtta |
|
| 179 |
7621 atagggtttt cttcaaataa ttgattgtag gtttgtacca actggataaa tagtttgtcc |
|
| 180 |
7681 acatcactat tatcaggatt taaatctccc tcaatcaaaa aatgaccacg aaacttaatc |
|
| 181 |
7741 atatgcgcta aggccaaata gattaagcgc aaatccgctt tatcagtaga atctaccaat |
|
| 182 |
7801 ttttttcgca gatgatagat agttggatat ttctcatgat aagcaacttc atctactata |
|
| 183 |
7861 tttccaaaaa taggatgacg ttcatgcttc ttgtcttctt ccaccaaaaa agactcttca |
|
| 184 |
7921 agtcgatgaa agaaactatc atctactttc gccatctcat ttgaaaaaat ctcctgtaga |
|
| 185 |
7981 taacaaatac gattcttccg acgtgtatac cttctacgag ctgtccgttt gagacgagtc |
|
| 186 |
8041 gcttccgctg tctctccact gtcaaataaa agagccccta taagattttt tttgatactg |
|
| 187 |
8101 tggcggtctg tatttcccag aaccttgaac tttttagacg gaaccttata atcatcagtg |
|
| 188 |
8161 atcaccgccc atccgacgct atttgtgccg atatctaagc ctattgagta tttcttatcc |
|
| 189 |
8221 atttttgcct cctaacttaa gaataagatc ttgtctcaac tgtataccga aatcagctca |
|
| 190 |
8281 ttaaaatcgc tttttttacc ataggttccg gtaataaagg catttttccc tataacaaaa |
|
| 191 |
8341 aaagcaagga ataatccctg cttttaataa tccaaatgag ataaaaatgt catgacattg |
|
| 192 |
8401 gtgtacagaa |
|
| 193 |
// |
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| ... |
No newline at end of file |
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0 comments (0 inline, 0 general)