diff --git a/README.md b/README.md
--- a/README.md
+++ b/README.md
@@ -21,7 +21,7 @@ Trp(-), Zeocin resistant, Chloramphenico
 
 ### Comments
 
-168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). 
+168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). GFP was inserted in _amyE_ using plasmid from Reference (2).
 
 ### References