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 This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus
 
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 ----------------- | ----------------- 
-**strain**        | _B. subtilis_ 168
-**synonyms**      | 168
-**parents**       | None
+**strain**        | _B. subtilis_ 168-GFP
+**synonyms**      | 168-GFP
+**parents**       | 168 wild type
 **obtained from** | ICOS lab
 **owner**         | Jonathan Tellechea
 
 ### Genotype
 
-_trpC2_, _BC659_
+_trpC2_, _BC207-ble_, _amyE::GFP-cat_
 
 ### Phenotype
 
-Trp(-)
+Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. 
 
 ### Comments
 
-Wild type strain used in this study. Barcode 659 present in barcoding site between _ybxH_ and _ybxI_ genes. 
+168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). 
 
 ### References
 
-1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
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+1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
+2. 2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23.
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