LOCUS pBS-CreLox-Vector 4456 bp ds-DNA circular 11-DEC-2019 DEFINITION . ACCESSION pTarget SOURCE Unknown. ORGANISM Unknown Unclassified. FEATURES Location/Qualifiers rep_origin join(4455..4456,1..681) /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2399..2895 /label="Right Homology" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 2399..3398 /label="Right Homology" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1887..1920 /label="lox71" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1396..1880 /label="Left Homology" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2365..2398 /label="lox66" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 883..1880 /label="Left homology" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 1887..2398 /label="lox71-zeocin-lox66" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 3565..4357 /label="aadA_spec/strep_resistance" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 75..663 /label="pMB1" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature complement(3583..3603) /label="SEQ and PCR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 ORIGIN 1 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 61 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcaagaagat ttcacgcaat 901 ggtgtgccgc tgtatggcac aattgcggtg ctgatcggtt tggctgtcgg cgttgtactg 961 aactatatcg cgccgccgaa gatctttgtc tatgtataca gcgcaagcgt gctcccaggg 1021 atgattcctt ggtttatcat tttgatcagc catatcggat tcagaaaagc gaagggtgct 1081 gcattagata aacatccatt caaaatgccg tttgctcctt ttacaaacta tttgacgatt 1141 gctttcttgc tgatggttct ggttggcatg tggtttaacg atgatactcg tatctccctg 1201 attgtaggcg tgattttctt agcgcttgtg gtcattagtt attatgtgtt tgggattggc 1261 aaacgcacgc aggccaattt gacaaaaagc gaacaagcag ctgaataacg ataaaaaaga 1321 gacattcacg gatgtctctt tttttatttt tcgcgtatgg attttcccta ttttagcaag 1381 ctatagggta aagatagcag aaggaaaggt gttcataaaa tggatgttac actcggttat 1441 ttgcgcgaat cactttcgaa tcatctagaa aatgaagtct gccagcgtat ttgcaagaag 1501 atgctggcga agcgttatgc aaatgaagag gaatttgtaa aagacttaga tgataatgaa 1561 atgtcttttt tgaaccatgt attagaaaaa gaaattaagt atgcccagaa tgaacaggat 1621 caaaaacggg ccaaggaatt aaacgaggtg tatgaattac tgctttgaga aaggcgggta 1681 agaaatggga gcaattgaac gaagcggata tacatttcag ccggagttta gcgttgtccg 1741 tcaaaacgga gccatccatg tgtaccatca aggagaattt gtggaagaaa tcgaatttga 1801 atttaacggg gaatatcccg atcatgattt gattgaagag cttgtgaatc attattgttt 1861 tgagcatgag atttagagcc ACTAGTtacc gttcgtatag catacattat acgaagttat 1921 gtttttcatg aaagacttga tatggctttt tatatgtgtt actctacata cagaaaggag 1981 gaactaaata tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc 2041 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac 2101 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg 2161 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag 2221 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc 2281 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac 2341 ttcgtggccg aggagcagga ctgaataact tcgtatagca tacattatac gaacggtaat 2401 ttaggatgta atcaggccat actttttcag gatatccacg gtgatgtttt tggcttttgt 2461 gccggaatca tcgacattgg tgacaaagac ataagagcca tgctctgttt tgatgaatcc 2521 gacaaaccat cctaatccca tatcagtcag gcgggttccg gttttcccgt agagcgtgta 2581 atgatcgcct tcctcttgaa tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa 2641 tggaagctcc tctttagcca gtttctccaa aaaagtttct tgttccagcg gagatattgt 2701 gagcgagctt tgcagccaaa actggtcgat tcccccgctg atatcctcgt tgccgtaaga 2761 tagagtatga agccatgttt tcattctttc ctcgccgata tctctcgcta atgcctgata 2821 ataccagatt gcggattcgc gcatggccga gccaagcgta tggtcgcggt tccatgattc 2881 gaattcacgc ttgaccccgt cccatcgttt aacatcgtat tcatctctta cagctttgac 2941 ctgcaaaccg attaaagcgt tcacgacttt aaaggtggat tgcggagttt gtctttgatt 3001 tgcccgtttt ctgttataga caaatgtctg atccttttgt aaatcgtgaa gaatgaaggt 3061 tccatccgta tccttgaatt catcatcgac attcattttg ctgacattca agtgtttttc 3121 atgagcagat gatgctgcgt ggacggagaa gccgccgatc cctgcaatac tcagcacaag 3181 cacaacatat atccattttt tcattttgtt ctccccttcg ttttgattaa ctaccttttc 3241 tctctttcgt ttgttcttcc tttaaaaaat aatcaaaaag cgcttaaatt tggttttgca 3301 aacaaatact gttactgggt ttatagtcgg aagataacgt gaaaatagag ggtaaaaagg 3361 aaggcagggt aagcggatga atgagcagat tccacatggt cgacctgcag aagcttagat 3421 ctattaccct gttatcccta ctcgagttca tgtgcagctc catcagcaaa aggggatgat 3481 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg 3541 tcatcagcgg tggagtgcaa tgtcatgagg gaagcggtga tcgccgaagt atcgactcaa 3601 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat 3661 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt 3721 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa 3781 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg 3841 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg 3901 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct 3961 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa 4021 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta 4081 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc 4141 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca 4201 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt 4261 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac 4321 gtgaaaggcg agatcaccaa ggtagtcggc aaataagatg ccgctcgcca gtcgattggc 4381 tgagctcatg aagttcctat tccgaagttc cgcgaacgcg taaaggatct aggtgaagat 4441 cctttttgat aatctc //