diff --git a/pEC-Vector.gb b/pEC-Vector.gb new file mode 100644 --- /dev/null +++ b/pEC-Vector.gb @@ -0,0 +1,123 @@ +LOCUS pEC-Vector 3777 bp ds-DNA circular 11-DEC-2019 +DEFINITION . +ACCESSION pTarget +SOURCE Unknown. + ORGANISM Unknown Unclassified. +FEATURES Location/Qualifiers + CDS complement(2531..2971) + /label="cat CDS" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + gene complement(2972..3190) + /label="cat gene" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + misc_feature 2080..2522 + /label="left_homology" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + misc_feature 3301..3334 + /label="FRT" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + CDS complement(2972..3190) + /label="cat CDS" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + -35_signal complement(3280..3285) + /label="cat promoter (counter-clockwise); ACGTAA" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + rep_origin 151..532 + /label="rep_origin" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + promoter complement(1838..1866) + /label="amp prom" + /ApEinfo_revcolor=#ff9ccd + /ApEinfo_fwdcolor=#ff9ccd + -10_signal complement(3257..3262) + /label="cat promoter (counter-clockwise); CATATT" + /ApEinfo_revcolor=#9eafd2 + /ApEinfo_fwdcolor=#9eafd2 + misc_feature 1996..1997 + /label="Geneious type: origin" + /ApEinfo_revcolor=#c6c9d1 + /ApEinfo_fwdcolor=#c6c9d1 + marker complement(936..1796) + /label="amp marker" + /ApEinfo_revcolor=#f8d3a9 + /ApEinfo_fwdcolor=#f8d3a9 + misc_feature 3335..3777 + /label="right_homology" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 + misc_feature 3304..3331 + /label="overlap" + /ApEinfo_revcolor=#b4abac + /ApEinfo_fwdcolor=#b4abac +ORIGIN + 1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg + 61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca + 121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta + 181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac + 241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg + 301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat + 361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt + 421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag + 481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac + 541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac + 601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc + 661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt + 721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc + 781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg + 841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca + 901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca + 961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag + 1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac + 1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc + 1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct + 1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc + 1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg + 1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc + 1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat + 1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag + 1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat + 1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg + 1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca + 1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga + 1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc + 1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata + 1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg + 1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc + 1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag + 2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc + 2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt + 2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga + 2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc + 2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc + 2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa + 2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca + 2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg + 2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca + 2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca + 2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt + 2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga + 2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca + 2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg + 2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata + 2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg + 3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa + 3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg + 3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt + 3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg + 3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc + 3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac + 3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg + 3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga + 3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt + 3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt + 3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct + 3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc + 3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc +// \ No newline at end of file