# HG changeset patch # User Jonathan Tellechea # Date 2019-12-11 15:56:21 # Node ID e653989498f1de4b959b9e65c9fd007ca0d5deae # Parent fb92a852d5e3cdff50fc2d1b68da7affa92c628f Corrected version diff --git a/pEC-Vector.gb b/pEC-Vector.gb --- a/pEC-Vector.gb +++ b/pEC-Vector.gb @@ -1,61 +1,61 @@ -LOCUS pEC-Vector 3777 bp ds-DNA circular 11-DEC-2019 +LOCUS pEC-Vector 3811 bp ds-DNA circular 11-DEC-2019 DEFINITION . ACCESSION pTarget SOURCE Unknown. ORGANISM Unknown Unclassified. FEATURES Location/Qualifiers - CDS complement(2531..2971) - /label="cat CDS" - /ApEinfo_revcolor=#c7b0e3 - /ApEinfo_fwdcolor=#c7b0e3 - gene complement(2972..3190) - /label="cat gene" - /ApEinfo_revcolor=#75c6a9 - /ApEinfo_fwdcolor=#75c6a9 misc_feature 2080..2522 /label="left_homology" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 - misc_feature 3301..3334 + misc_feature 3369..3811 + /label="right_homology" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 + misc_feature 3335..3368 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 - CDS complement(2972..3190) + -10_signal complement(3291..3296) + /label="cat promoter (counter-clockwise); CATATT" + /ApEinfo_revcolor=#9eafd2 + /ApEinfo_fwdcolor=#9eafd2 + CDS complement(2565..3005) /label="cat CDS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 - -35_signal complement(3280..3285) + gene complement(3006..3224) + /label="cat gene" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + marker complement(936..1796) + /label="amp marker" + /ApEinfo_revcolor=#f8d3a9 + /ApEinfo_fwdcolor=#f8d3a9 + promoter complement(1838..1866) + /label="amp prom" + /ApEinfo_revcolor=#ff9ccd + /ApEinfo_fwdcolor=#ff9ccd + misc_feature 2530..2563 + /label="FRT" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 + -35_signal complement(3314..3319) /label="cat promoter (counter-clockwise); ACGTAA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 + misc_feature 1996..1997 + /label="Geneious type: origin" + /ApEinfo_revcolor=#c6c9d1 + /ApEinfo_fwdcolor=#c6c9d1 + CDS complement(3006..3224) + /label="cat CDS" + /ApEinfo_revcolor=#c7b0e3 + /ApEinfo_fwdcolor=#c7b0e3 rep_origin 151..532 /label="rep_origin" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 - promoter complement(1838..1866) - /label="amp prom" - /ApEinfo_revcolor=#ff9ccd - /ApEinfo_fwdcolor=#ff9ccd - -10_signal complement(3257..3262) - /label="cat promoter (counter-clockwise); CATATT" - /ApEinfo_revcolor=#9eafd2 - /ApEinfo_fwdcolor=#9eafd2 - misc_feature 1996..1997 - /label="Geneious type: origin" - /ApEinfo_revcolor=#c6c9d1 - /ApEinfo_fwdcolor=#c6c9d1 - marker complement(936..1796) - /label="amp marker" - /ApEinfo_revcolor=#f8d3a9 - /ApEinfo_fwdcolor=#f8d3a9 - misc_feature 3335..3777 - /label="right_homology" - /ApEinfo_revcolor=#faac61 - /ApEinfo_fwdcolor=#faac61 - misc_feature 3304..3331 - /label="overlap" - /ApEinfo_revcolor=#b4abac - /ApEinfo_fwdcolor=#b4abac ORIGIN 1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg 61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca @@ -99,25 +99,26 @@ ORIGIN 2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa 2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca 2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg - 2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca - 2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca - 2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt - 2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga - 2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca - 2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg - 2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata - 2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg - 3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa - 3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg - 3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt - 3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg - 3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc - 3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac - 3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg - 3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga - 3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt - 3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt - 3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct - 3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc - 3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc + 2521 cgtGGATCCg aagttcctat tctctagaaa gtataggaac ttcattacgc cccgccctgc + 2581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa + 2641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat + 2701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa + 2761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta + 2821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac + 2881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg + 2941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc + 3001 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa + 3061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc + 3121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat + 3181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct + 3241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga + 3301 aagttggaac ctcttacgtg ccgatcaacg tctcgaagtt cctattctct agaaagtata + 3361 ggaacttcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg + 3421 gaagccctat ttcttttatg gaatcagcag gctggaacct tgcgtcgccc ggctttccag + 3481 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac + 3541 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc + 3601 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt + 3661 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa + 3721 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta + 3781 caccacgcgc actttcttac cgccggtgat c // \ No newline at end of file