# HG changeset patch # User Jonathan Tellechea # Date 2019-12-11 15:20:11 # Node ID fb92a852d5e3cdff50fc2d1b68da7affa92c628f # Parent 125790a5143cb817cf390fe2e9fa56a08298dbfb Vector for CRISPR barcoding diff --git a/pEC-CRISPR-Vector.gb b/pEC-CRISPR-Vector.gb new file mode 100644 --- /dev/null +++ b/pEC-CRISPR-Vector.gb @@ -0,0 +1,129 @@ +LOCUS pEC-CRISPR-Vector 3178 bp ds-DNA circular 11-DEC-2019 +DEFINITION . +SOURCE Cloning vector pUC19 + ORGANISM Cloning vector pUC19 other sequences; artificial sequences; + vectors. +FEATURES Location/Qualifiers + misc_RNA complement(1359..1911) + /label="RNAII transcript (complementary strand)" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + gene complement(2118..2978) + /label="bla gene" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + CDS 1123..1162 + /label="spy-tem" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + -35_signal complement(3043..3048) + /label="bla promoter (counter-clockwise); TTCAAA" + /ApEinfo_revcolor=#85dae9 + /ApEinfo_fwdcolor=#85dae9 + rep_origin complement(1359..1947) + /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + -10_signal complement(3022..3027) + /label="bla promoter (counter-clockwise); GAGACA" + /ApEinfo_revcolor=#85dae9 + /ApEinfo_fwdcolor=#85dae9 + -35_signal 1765..1770 + /label="RNAI promoter (clockwise); TTGAAG" + /ApEinfo_revcolor=#f8d3a9 + /ApEinfo_fwdcolor=#f8d3a9 + misc_feature 535..977 + /label="right_homology" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 + CDS 1081..1122 + /label="SP_scaffold" + /ApEinfo_revcolor=#f58a5e + /ApEinfo_fwdcolor=#f58a5e + misc_RNA 1801..1908 + /label="RNAI transcript" + /ApEinfo_revcolor=#75c6a9 + /ApEinfo_fwdcolor=#75c6a9 + -35_signal complement(1942..1947) + /label="RNAII promoter (counter-clockwise); TTGAGA" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + -10_signal 1787..1792 + /label="RNAI promoter (clockwise); GCTACA" + /ApEinfo_revcolor=#b1ff67 + /ApEinfo_fwdcolor=#b1ff67 + -10_signal complement(1921..1926) + /label="RNAII promoter (counter-clockwise); CGTAAT" + /ApEinfo_revcolor=#d6b295 + /ApEinfo_fwdcolor=#d6b295 + misc_feature 84..526 + /label="left_homology" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + CDS 1026..1054 + /label="pJ23119" + /ApEinfo_revcolor=#ffef86 + /ApEinfo_fwdcolor=#ffef86 + sig_peptide complement(2910..2978) + /label="bla sig_peptide" + /ApEinfo_revcolor=#b7e6d7 + /ApEinfo_fwdcolor=#b7e6d7 + CDS complement(2118..2978) + /label="bla CDS" + /ApEinfo_revcolor=#faac61 + /ApEinfo_fwdcolor=#faac61 +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgtagtca aattcaccac gccctgcgca ccgtcgcggg + 121 gcgttttgct gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag + 181 atttcatatt tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat + 241 gctgatggtc accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc + 301 cattatgttc ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact + 361 ggcgattgcg gtggtgtggg ttattaaggc gattaaagca ccaaaagtgc cgaaatatca + 421 gcgttatgac cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat + 481 ccttagaaca aaataggaat tgataatcaa atctgtcact attgcgtGCA TGCagtgctg + 541 taccctacat acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct + 601 tttatggaat cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac + 661 gctgcgcatc cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc + 721 tggcaatcaa agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat + 781 agccttgcag ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt + 841 tcacaccggt aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca + 901 ggcaatccag cgaccgttcc caggtgtctc tgcccacgga atcgtacacc acgcgcactt + 961 tcttaccgcc ggtgatcttc tccttacgca tctgtgcggt atttcacacc gcatatgctg + 1021 gatccttgac agctagctca gtcctaggta taatactagt CAGCACGATG AATCTGTTAG + 1081 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt + 1141 ggcaccgagt cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta + 1201 ttaccctgtt atccctactc gagttcatgt gcagctccag ctcactcaaa ggcggtaata + 1261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa + 1321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct + 1381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa + 1441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg + 1501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca + 1561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa + 1621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg + 1681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg + 1741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga + 1801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc + 1861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag + 1921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac + 1981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc + 2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag + 2101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt + 2161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag + 2221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca + 2281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact + 2341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca + 2401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg + 2461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc + 2521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg + 2581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca + 2641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt + 2701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc + 2761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc + 2821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca + 2881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa + 2941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat + 3001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa + 3061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa + 3121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc +// \ No newline at end of file