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j.tellechea-luzardo@newcastle.ac.uk
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LOCUS pEC-CRISPR-Vector 3178 bp ds-DNA circular 11-DEC-2019 |
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DEFINITION . |
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SOURCE Cloning vector pUC19 |
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ORGANISM Cloning vector pUC19 other sequences; artificial sequences; |
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vectors. |
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FEATURES Location/Qualifiers |
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misc_RNA complement(1359..1911) |
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/label="RNAII transcript (complementary strand)" |
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/ApEinfo_revcolor=#b7e6d7 |
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/ApEinfo_fwdcolor=#b7e6d7 |
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gene complement(2118..2978) |
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/label="bla gene" |
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/ApEinfo_revcolor=#75c6a9 |
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/ApEinfo_fwdcolor=#75c6a9 |
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CDS 1123..1162 |
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/label="spy-tem" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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-35_signal complement(3043..3048) |
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/label="bla promoter (counter-clockwise); TTCAAA" |
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/ApEinfo_revcolor=#85dae9 |
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/ApEinfo_fwdcolor=#85dae9 |
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rep_origin complement(1359..1947) |
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/label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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-10_signal complement(3022..3027) |
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/label="bla promoter (counter-clockwise); GAGACA" |
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/ApEinfo_revcolor=#85dae9 |
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/ApEinfo_fwdcolor=#85dae9 |
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-35_signal 1765..1770 |
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/label="RNAI promoter (clockwise); TTGAAG" |
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/ApEinfo_revcolor=#f8d3a9 |
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/ApEinfo_fwdcolor=#f8d3a9 |
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misc_feature 535..977 |
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/label="right_homology" |
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/ApEinfo_revcolor=#faac61 |
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/ApEinfo_fwdcolor=#faac61 |
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CDS 1081..1122 |
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/label="SP_scaffold" |
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/ApEinfo_revcolor=#f58a5e |
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/ApEinfo_fwdcolor=#f58a5e |
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misc_RNA 1801..1908 |
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/label="RNAI transcript" |
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/ApEinfo_revcolor=#75c6a9 |
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/ApEinfo_fwdcolor=#75c6a9 |
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-35_signal complement(1942..1947) |
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/label="RNAII promoter (counter-clockwise); TTGAGA" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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-10_signal 1787..1792 |
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/label="RNAI promoter (clockwise); GCTACA" |
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/ApEinfo_revcolor=#b1ff67 |
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/ApEinfo_fwdcolor=#b1ff67 |
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-10_signal complement(1921..1926) |
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/label="RNAII promoter (counter-clockwise); CGTAAT" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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misc_feature 84..526 |
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/label="left_homology" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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CDS 1026..1054 |
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/label="pJ23119" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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sig_peptide complement(2910..2978) |
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/label="bla sig_peptide" |
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/ApEinfo_revcolor=#b7e6d7 |
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/ApEinfo_fwdcolor=#b7e6d7 |
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CDS complement(2118..2978) |
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/label="bla CDS" |
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/ApEinfo_revcolor=#faac61 |
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/ApEinfo_fwdcolor=#faac61 |
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ORIGIN |
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1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca |
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61 cagcttgtct gtaagcggat gccgtagtca aattcaccac gccctgcgca ccgtcgcggg |
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121 gcgttttgct gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag |
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181 atttcatatt tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat |
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241 gctgatggtc accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc |
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301 cattatgttc ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact |
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361 ggcgattgcg gtggtgtggg ttattaaggc gattaaagca ccaaaagtgc cgaaatatca |
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421 gcgttatgac cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat |
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481 ccttagaaca aaataggaat tgataatcaa atctgtcact attgcgtGCA TGCagtgctg |
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541 taccctacat acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct |
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601 tttatggaat cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac |
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661 gctgcgcatc cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc |
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721 tggcaatcaa agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat |
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781 agccttgcag ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt |
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841 tcacaccggt aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca |
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901 ggcaatccag cgaccgttcc caggtgtctc tgcccacgga atcgtacacc acgcgcactt |
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961 tcttaccgcc ggtgatcttc tccttacgca tctgtgcggt atttcacacc gcatatgctg |
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1021 gatccttgac agctagctca gtcctaggta taatactagt CAGCACGATG AATCTGTTAG |
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1081 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt |
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1141 ggcaccgagt cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta |
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1201 ttaccctgtt atccctactc gagttcatgt gcagctccag ctcactcaaa ggcggtaata |
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1261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa |
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1321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct |
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1381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa |
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1441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg |
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1501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca |
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1561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa |
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1621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg |
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1681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg |
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1741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga |
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1801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc |
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1861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag |
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1921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac |
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1981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc |
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2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag |
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2101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt |
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2161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag |
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2221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca |
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2281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact |
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2341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca |
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2401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg |
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2461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc |
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2521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg |
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2581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca |
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2641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt |
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2701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc |
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2761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc |
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2821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca |
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2881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa |
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2941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat |
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3001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa |
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3061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa |
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3121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc |
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// |
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No newline at end of file |
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