LOCUS pEC-Vector 3777 bp ds-DNA circular 11-DEC-2019 DEFINITION . ACCESSION pTarget SOURCE Unknown. ORGANISM Unknown Unclassified. FEATURES Location/Qualifiers CDS complement(2531..2971) /label="cat CDS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 gene complement(2972..3190) /label="cat gene" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2080..2522 /label="left_homology" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 3301..3334 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 CDS complement(2972..3190) /label="cat CDS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 -35_signal complement(3280..3285) /label="cat promoter (counter-clockwise); ACGTAA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin 151..532 /label="rep_origin" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 promoter complement(1838..1866) /label="amp prom" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd -10_signal complement(3257..3262) /label="cat promoter (counter-clockwise); CATATT" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1996..1997 /label="Geneious type: origin" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 marker complement(936..1796) /label="amp marker" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 3335..3777 /label="right_homology" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 3304..3331 /label="overlap" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac ORIGIN 1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg 61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta 181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac 241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg 301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat 361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt 421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag 481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac 541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc 2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt 2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga 2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc 2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc 2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa 2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca 2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg 2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca 2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca 2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt 2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga 2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca 2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg 2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata 2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg 3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa 3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg 3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt 3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg 3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc 3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac 3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg 3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga 3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt 3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt 3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct 3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc 3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc //