LOCUS pEC-Vector 3811 bp ds-DNA circular 11-DEC-2019 DEFINITION . ACCESSION pTarget SOURCE Unknown. ORGANISM Unknown Unclassified. FEATURES Location/Qualifiers misc_feature 2080..2522 /label="left_homology" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 3369..3811 /label="right_homology" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 3335..3368 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 -10_signal complement(3291..3296) /label="cat promoter (counter-clockwise); CATATT" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(2565..3005) /label="cat CDS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 gene complement(3006..3224) /label="cat gene" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 marker complement(936..1796) /label="amp marker" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter complement(1838..1866) /label="amp prom" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2530..2563 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 -35_signal complement(3314..3319) /label="cat promoter (counter-clockwise); ACGTAA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1996..1997 /label="Geneious type: origin" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(3006..3224) /label="cat CDS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin 151..532 /label="rep_origin" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg 61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta 181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac 241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg 301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat 361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt 421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag 481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac 541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc 2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt 2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga 2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc 2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc 2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa 2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca 2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg 2521 cgtGGATCCg aagttcctat tctctagaaa gtataggaac ttcattacgc cccgccctgc 2581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa 2641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat 2701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa 2761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta 2821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac 2881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg 2941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc 3001 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa 3061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc 3121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat 3181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct 3241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga 3301 aagttggaac ctcttacgtg ccgatcaacg tctcgaagtt cctattctct agaaagtata 3361 ggaacttcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg 3421 gaagccctat ttcttttatg gaatcagcag gctggaacct tgcgtcgccc ggctttccag 3481 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac 3541 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc 3601 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt 3661 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa 3721 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta 3781 caccacgcgc actttcttac cgccggtgat c //