LOCUS pEC-CRISPR-Vector 3178 bp ds-DNA circular 11-DEC-2019 DEFINITION . SOURCE Cloning vector pUC19 ORGANISM Cloning vector pUC19 other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers misc_RNA complement(1359..1911) /label="RNAII transcript (complementary strand)" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 gene complement(2118..2978) /label="bla gene" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 CDS 1123..1162 /label="spy-tem" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 -35_signal complement(3043..3048) /label="bla promoter (counter-clockwise); TTCAAA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 rep_origin complement(1359..1947) /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 -10_signal complement(3022..3027) /label="bla promoter (counter-clockwise); GAGACA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 -35_signal 1765..1770 /label="RNAI promoter (clockwise); TTGAAG" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 535..977 /label="right_homology" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 1081..1122 /label="SP_scaffold" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_RNA 1801..1908 /label="RNAI transcript" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 -35_signal complement(1942..1947) /label="RNAII promoter (counter-clockwise); TTGAGA" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 -10_signal 1787..1792 /label="RNAI promoter (clockwise); GCTACA" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 -10_signal complement(1921..1926) /label="RNAII promoter (counter-clockwise); CGTAAT" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 84..526 /label="left_homology" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 1026..1054 /label="pJ23119" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 sig_peptide complement(2910..2978) /label="bla sig_peptide" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS complement(2118..2978) /label="bla CDS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgtagtca aattcaccac gccctgcgca ccgtcgcggg 121 gcgttttgct gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag 181 atttcatatt tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat 241 gctgatggtc accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc 301 cattatgttc ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact 361 ggcgattgcg gtggtgtggg ttattaaggc gattaaagca ccaaaagtgc cgaaatatca 421 gcgttatgac cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat 481 ccttagaaca aaataggaat tgataatcaa atctgtcact attgcgtGCA TGCagtgctg 541 taccctacat acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct 601 tttatggaat cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac 661 gctgcgcatc cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc 721 tggcaatcaa agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat 781 agccttgcag ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt 841 tcacaccggt aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca 901 ggcaatccag cgaccgttcc caggtgtctc tgcccacgga atcgtacacc acgcgcactt 961 tcttaccgcc ggtgatcttc tccttacgca tctgtgcggt atttcacacc gcatatgctg 1021 gatccttgac agctagctca gtcctaggta taatactagt CAGCACGATG AATCTGTTAG 1081 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt 1141 ggcaccgagt cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta 1201 ttaccctgtt atccctactc gagttcatgt gcagctccag ctcactcaaa ggcggtaata 1261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 2401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 2461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 2761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 3121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc //