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j.tellechea-luzardo@newcastle.ac.uk
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# B.subtilis-GFP |
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This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus |
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**strain** | _B. subtilis_ 168-GFP |
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**synonyms** | 168-GFP |
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**parents** | 168 wild type |
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**obtained from** | ICOS lab |
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**owner** | Jonathan Tellechea |
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### Genotype |
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_trpC2_, _BC207-ble_, _amyE::GFP-cat_ |
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### Phenotype |
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Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. |
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### Comments |
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168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). |
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168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). GFP was inserted in _amyE_ using plasmid from Reference (2). |
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### References |
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1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8. |
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2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23. |
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