hg B.subtilis-GFP

Summary

Description:

GFP expression experiments in B.subtilis

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Latest Changes

Revision	Commit Message	Age	Author	Barcode
r12:84fd403c44e6	Vector used to barcode B.subtilis using CRISPR	5 years ago	jtl	default
r11:9117870bc39b	Vector used to barcode B.subtilis using CreLox	5 years ago	jtl	default
r10:acc4afc32349	Edited file README.md via cellrepo	5 years and 5 months ago	jtl	CACAATCCTACCCTAAAGGTAATGGTTTCAACACTATAATCGCAAGAGTTCCAATGTGGTCTATATAGATGTTCAATAGTCCTAATCGTGCTACACGCATAGACTAACAGACATACCCAACTC default
r9:e354fbd67be2	Typo correction	5 years and 5 months ago	jtl	default
r8:00cabd8c81af	168-GFP strain description.	5 years and 5 months ago	jtl	default
r7:a507a4390920	Sequencing results of barcode 207 inserted in the GFP Strain.	5 years and 5 months ago	jtl	default
r6:de53449578d0	Figure showing the fluorescence emission after insertion.	5 years and 5 months ago	jtl	default
r5:7b624806c6cb	GenBank file of the integrative plasmid used to insert GFP in amyE locus.	5 years and 5 months ago	jtl	default
r4:070c713ad448	Sequencing result of Barcode 659 in strain 168	5 years and 5 months ago	jtl	CTTACTATGCCACATAGTCTAACTACTTTGCGTAGGTAATAGAGCAACGAGTAGGGTTATCGTCTTTCATACTCGGTTGGCGATTATACAAACTCCAATGGTTTGATTGGTGTTTCTCATACG default
r3:6bc7120e50bd	B. subtilis transformation protocol	5 years and 5 months ago	jtl	default

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README.md

B.subtilis-GFP

This repository describes how to get GFP inserted in Bacillus subtilis 168 amyE locus


strain	B. subtilis 168-GFP
synonyms	168-GFP
parents	168 wild type
obtained from	ICOS lab
owner	Jonathan Tellechea

Genotype

trpC2, BC207-ble, amyE::GFP-cat

Phenotype

Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission.

Comments

168-GFP emitting strain. Barcode 207 present in barcoding site between ybxH and ybxI genes (Zeocin resistant gene was kept next to the barcode sequence). GFP was inserted in amyE using plasmid from Reference (2).

References

BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23.