GFP expression experiments in B.subtilis
| Revision | Commit Message | Age | Author | Barcode | ||
|---|---|---|---|---|---|---|
|
|
r12:84fd403c44e6 | 4 years and 11 months ago | jtl | |||
|
|
r11:9117870bc39b | 4 years and 11 months ago | jtl | |||
|
|
r10:acc4afc32349 | 5 years and 4 months ago | jtl | |||
|
|
r9:e354fbd67be2 | 5 years and 4 months ago | jtl | |||
|
|
r8:00cabd8c81af | 5 years and 4 months ago | jtl | |||
|
|
r7:a507a4390920 | 5 years and 4 months ago | jtl | |||
|
|
r6:de53449578d0 | 5 years and 4 months ago | jtl | |||
|
|
r5:7b624806c6cb | 5 years and 4 months ago | jtl | |||
|
|
r4:070c713ad448 | 5 years and 4 months ago | jtl | |||
|
|
r3:6bc7120e50bd | 5 years and 4 months ago | jtl |
B.subtilis-GFP
This repository describes how to get GFP inserted in Bacillus subtilis 168 amyE locus
| strain | B. subtilis 168-GFP |
| synonyms | 168-GFP |
| parents | 168 wild type |
| obtained from | ICOS lab |
| owner | Jonathan Tellechea |
Genotype
trpC2, BC207-ble, amyE::GFP-cat
Phenotype
Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission.
Comments
168-GFP emitting strain. Barcode 207 present in barcoding site between ybxH and ybxI genes (Zeocin resistant gene was kept next to the barcode sequence). GFP was inserted in amyE using plasmid from Reference (2).
References
- BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
- Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23.