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j.tellechea-luzardo@newcastle.ac.uk
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@@ -18,12 +18,12 @@ This repository describes how to get GFP |
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### Phenotype |
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Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. |
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### Comments |
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168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). |
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168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). GFP was inserted in _amyE_ using plasmid from Reference (2). |
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### References |
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1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8. |
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2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23. |
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