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Jonathan Tellechea (jtl) - 5 years ago 2019-07-03 15:16:36
j.tellechea-luzardo@newcastle.ac.uk
168-GFP strain description.
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This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus
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**strain**        | _B. subtilis_ 168
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**synonyms**      | 168
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**parents**       | None
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**strain**        | _B. subtilis_ 168-GFP
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**synonyms**      | 168-GFP
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**parents**       | 168 wild type
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**obtained from** | ICOS lab
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**owner**         | Jonathan Tellechea
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### Genotype
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_trpC2_, _BC659_
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_trpC2_, _BC207-ble_, _amyE::GFP-cat_
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### Phenotype
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Trp(-)
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Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. 
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### Comments
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Wild type strain used in this study. Barcode 659 present in barcoding site between _ybxH_ and _ybxI_ genes. 
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168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). 
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### References
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1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
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1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.
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2. 2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23.
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