0
1
0
j.tellechea-luzardo@newcastle.ac.uk
1 file changed with 9 insertions and 7 deletions:
9
7
| 1 | 1 |
# B.subtilis-GFP |
| 2 | 2 |
|
| 3 | 3 |
This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus |
| 4 | 4 |
|
| 5 |
|
|
| 5 | 6 |
| |
| 6 | 7 |
----------------- | ----------------- |
| 7 |
**strain** | _B. subtilis_ 168 |
|
| 8 |
**synonyms** | 168 |
|
| 9 |
** |
|
| 8 |
**strain** | _B. subtilis_ 168-GFP |
|
| 9 |
**synonyms** | 168-GFP |
|
| 10 |
**parents** | 168 wild type |
|
| 10 | 11 |
**obtained from** | ICOS lab |
| 11 | 12 |
**owner** | Jonathan Tellechea |
| 12 | 13 |
|
| 13 | 14 |
### Genotype |
| 14 | 15 |
|
| 15 |
_trpC2_, |
|
| 16 |
_trpC2_, _BC207-ble_, _amyE::GFP-cat_ |
|
| 16 | 17 |
|
| 17 | 18 |
### Phenotype |
| 18 | 19 |
|
| 19 |
Trp(-) |
|
| 20 |
Trp(-), Zeocin resistant, Chloramphenicol resistant, GFP emission. |
|
| 20 | 21 |
|
| 21 | 22 |
### Comments |
| 22 | 23 |
|
| 23 |
|
|
| 24 |
168-GFP emitting strain. Barcode 207 present in barcoding site between _ybxH_ and _ybxI_ genes (Zeocin resistant gene was kept next to the barcode sequence). |
|
| 24 | 25 |
|
| 25 | 26 |
### References |
| 26 | 27 |
|
| 27 |
1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8. |
|
| ... |
No newline at end of file |
|
| 28 |
1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8. |
|
| 29 |
2. 2. Jan-Willem Veening, Heath Murray and Jeff Errington. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes Dev. 2009, 23. |
|
| ... |
No newline at end of file |
|
0 comments (0 inline, 0 general)