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Jonathan Tellechea (jtl) - 5 years ago 2019-07-03 15:00:48
j.tellechea-luzardo@newcastle.ac.uk
GenBank file of the integrative plasmid used to insert GFP in amyE locus.
1 file changed with 174 insertions and 0 deletions:
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new file 100644
1
 
LOCUS       pGFP-rrnB               8009 bp ds-DNA     circular     08-NOV-2018
2
 
DEFINITION  .
3
 
ACCESSION   pGFP                                                               
4
 
SOURCE      Unknown.                                                           
5
 
  ORGANISM  Unknown Unclassified.                                              
6
 
FEATURES             Location/Qualifiers
7
 
     CDS             6345..7052
8
 
                     /label="gfp' CDS"
9
 
                     /ApEinfo_revcolor=#d59687
10
 
                     /ApEinfo_fwdcolor=#d59687
11
 
     misc_feature    complement(7173..7259)
12
 
                     /label="ter misc_feature"
13
 
                     /ApEinfo_revcolor=#b4abac
14
 
                     /ApEinfo_fwdcolor=#b4abac
15
 
     misc_feature    complement(7078..7166)
16
 
                     /label="(null) misc_feature"
17
 
                     /ApEinfo_revcolor=#b7e6d7
18
 
                     /ApEinfo_fwdcolor=#b7e6d7
19
 
     CDS             199..1071
20
 
                     /label="bla CDS"
21
 
                     /ApEinfo_revcolor=#ffef86
22
 
                     /ApEinfo_fwdcolor=#ffef86
23
 
     CDS             complement(2077..2892)
24
 
                     /label="spc CDS"
25
 
                     /ApEinfo_revcolor=#b7e6d7
26
 
                     /ApEinfo_fwdcolor=#b7e6d7
27
 
     CDS             complement(7375..7895)
28
 
                     /label="amyE' CDS"
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                     /ApEinfo_revcolor=#ff9ccd
30
 
                     /ApEinfo_fwdcolor=#ff9ccd
31
 
     CDS             complement(5367..6029)
32
 
                     /label="cat CDS"
33
 
                     /ApEinfo_revcolor=#f58a5e
34
 
                     /ApEinfo_fwdcolor=#f58a5e
35
 
     CDS             complement(3968..5005)
36
 
                     /label="'amyE CDS"
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                     /ApEinfo_revcolor=#f8d3a9
38
 
                     /ApEinfo_fwdcolor=#f8d3a9
39
 
ORIGIN
40
 
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
41
 
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
42
 
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
43
 
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
44
 
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
45
 
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
46
 
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
47
 
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
48
 
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
49
 
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
50
 
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
51
 
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
52
 
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
53
 
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
54
 
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
55
 
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
56
 
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
57
 
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
58
 
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
59
 
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
60
 
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
61
 
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
62
 
     1321 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
63
 
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
64
 
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
65
 
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
66
 
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
67
 
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
68
 
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
69
 
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
70
 
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct
71
 
     1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg
72
 
     1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat
73
 
     1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc
74
 
     2041 agaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga
75
 
     2101 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc
76
 
     2161 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa
77
 
     2221 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt
78
 
     2281 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat
79
 
     2341 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag
80
 
     2401 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac
81
 
     2461 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct
82
 
     2521 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact
83
 
     2581 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga
84
 
     2641 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac
85
 
     2701 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa
86
 
     2761 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat
87
 
     2821 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gttgattatg
88
 
     2881 ttcatataaa gtttatattg atactcaatt tacttaccct agattggaca tatacttaaa
89
 
     2941 ttactgttca ataaagctga ccgttagcgt ttaagtacat cctttcacaa tttgtctaca
90
 
     3001 gattaataat tattctttat tatacagatc gatcctctag acctaggcct taagatctga
91
 
     3061 tcatatgcat ccgcgggccc gggttaacgc gtaatccatg gatcaagaga caggatgagg
92
 
     3121 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggtgc cctgaatgaa
93
 
     3181 ctgcagaaag agctggtagt tggcgcactg ttcgaagaac tgccgatgtc cagtaagatt
94
 
     3241 cttactatgc tggttgaacc ggatgctggt aaagctactt gggttgctgc ttctacttat
95
 
     3301 ggtaccgata caactactgg tgaggaagtt aaaggagctc ttaaagaaat ccacttcagt
96
 
     3361 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
97
 
     3421 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
98
 
     3481 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
99
 
     3541 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
100
 
     3601 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgacta
101
 
     3661 agaaaatgcc gtcaaatccg ctcgccatga cttcactaac gatgcctttg aaaatcttca
102
 
     3721 agttcttttc tactaattca aggcgtgtct caccaggttt ttggtttgct ccggcgcaaa
103
 
     3781 tgcagacaat atcagcatcc ttgcagggta tgtttctctt tgatgtcttt ttgtttgtga
104
 
     3841 agtatttcac atttatattg tgcaacactt cacaaacttt tgcaagagaa aagttttgtc
105
 
     3901 tgatttatga acaaaaaaga aaccatcatt gatggtttct ttcggtaagt cccgtctagc
106
 
     3961 cttgccctca atggggaaga gaaccgctta agcccgagtc attatataaa ccatttagca
107
 
     4021 cgtaatcaaa gccaggctga ttctgaccgg gcacttgggc gctgccatta ttaaaaatca
108
 
     4081 cttttgcgtt ggttgtatcc gtgtccgcag gcagcgtcag cgtgtaaatt ccgtctgcat
109
 
     4141 ttttagtcat tggttttcca ggccaagatc cggtcaattc aattactcgg ctcccatcat
110
 
     4201 gtttatagat ataagcattt acctggctcc aatgattcgg attttgatag ccgatggttt
111
 
     4261 tggccgacgc tggatctctt ttaacaaaac tgtatttctc ggtcctcgtt acaccatcac
112
 
     4321 tgttcgttcc ttttaacatg atggtgtatg ttttgccaaa ttggatctcc ttttccgatt
113
 
     4381 gtgaattgat ctccatcctt aaacgcctgt cgtctggtcc attattgatt tgataaacgg
114
 
     4441 cttttgttgt attcgcatct gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg
115
 
     4501 ttacacctgt tttgtaattc tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat
116
 
     4561 aaagcacagc tacagacctg gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa
117
 
     4621 atgaacccgc tccagcttta ttgtcatacc tgccatcagg caattttgtt gccgtattga
118
 
     4681 tagagacaga ggatgaacct gcatttgcca gcacaacgcc atgtgagccg cgctgattca
119
 
     4741 taaatatctg gttgtttcca ttcgggttcg agagttcctc aggctgtcca gccatcacat
120
 
     4801 tgtgaaatct attgaccgca gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc
121
 
     4861 ctatttggct tttccccggg aacctcacac catttccgcc tccctcaggt ctggaaaaga
122
 
     4921 aaagaggcgt actgcctgaa cgagaagcta tcaccgccca gcctaaacgg atatcatcat
123
 
     4981 cgctcatcca tgtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
124
 
     5041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
125
 
     5101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
126
 
     5161 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
127
 
     5221 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggagctg
128
 
     5281 taatataaaa accttcttca actaacgggg caggttagtg acattagaaa accgactgta
129
 
     5341 aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat
130
 
     5401 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc
131
 
     5461 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata
132
 
     5521 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc
133
 
     5581 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc
134
 
     5641 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca
135
 
     5701 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa
136
 
     5761 agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa
137
 
     5821 gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat
138
 
     5881 ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa
139
 
     5941 gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca
140
 
     6001 attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg
141
 
     6061 cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa
142
 
     6121 cataaatata tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg
143
 
     6181 gtgctttagt tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa
144
 
     6241 ggatttgagc gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg
145
 
     6301 ccgatgataa gctgtcaaac atgagaattc gcggccgcgc tagcaaagga gaagaacttt
146
 
     6361 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
147
 
     6421 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
148
 
     6481 gcactactgg aaaactacct gttccatggc caacacttgt cactactttg acctatggtg
149
 
     6541 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
150
 
     6601 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
151
 
     6661 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
152
 
     6721 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
153
 
     6781 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
154
 
     6841 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
155
 
     6901 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
156
 
     6961 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
157
 
     7021 ggattacaca tggcatggat gagctctaca aataataagg ccggccaata aagatcctag
158
 
     7081 gacgccgcca agccagctta aacccagctc aatgagctgg gttttttgtt tgttaaaaat
159
 
     7141 gaagaagaaa ctgtgaagcg tatttaacta gtaaaatcta agacatatca tgatttataa
160
 
     7201 aaacaaaaaa accttgcagg atatgcaagg cttctggatg acccgtacgg gattcgaact
161
 
     7261 ccggatgtga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
162
 
     7321 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatcgatca
163
 
     7381 gaccagtttt taatttgtgt gtttccatgt gtccagtttg gaatactctt aacctcattg
164
 
     7441 gaaatcgcgg cataatcact ggtggtatga ttgatgaccg cgtcaacaat gacctttatg
165
 
     7501 ccatattctt cagcggctgc acacatttct ttaaattctt gttcagtacc taagtaacgg
166
 
     7561 ttgccaattt gatacgatgt cggctgatac agccagtacc agttcgacat gcttttatct
167
 
     7621 ccttgattcc cttcctttac ttggttaatc ggagatgtct gaatggctgt atatcctgca
168
 
     7681 tcatgaatat ccttcatatt gtgttttaac gtattgaacg accaattcca tgcatgaaga
169
 
     7741 atggttccgc ttttgatcga cggtgctgta agctcattcg atttgttcgc cgtttcagca
170
 
     7801 ctcgcagccg ccggtcctgc cagaaccaaa tgaaacagca ataaaaatcc agcgaataac
171
 
     7861 ggcagtaaag aggttttgaa tcgttttgca aacattcttg acactcctta tttgattttt
172
 
     7921 tgaagactta cttcggagtc aaaaatccct cttacttcat tcttccgctt cctcctttca
173
 
     7981 aaccgatgtg aagactggag aattttgtt
174
 
//
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