# B.subtilis-GFP

This repository describes how to get GFP inserted in _Bacillus subtilis_ 168 _amyE_ locus

 | 
----------------- | ----------------- 
**strain**        | _B. subtilis_ 168
**synonyms**      | 168
**parents**       | None
**obtained from** | ICOS lab
**owner**         | Jonathan Tellechea

### Genotype

_trpC2_, _BC659_

### Phenotype

Trp(-)

### Comments

Wild type strain used in this study. Barcode 659 present in barcoding site between _ybxH_ and _ybxI_ genes. 

### References

1. BURKHOLDER PR, GILES NH Jr. Induced biochemical mutations in Bacillus subtilis. Am J Bot. 1947 Jun; 34(6):345-8.