Location: B.subtilis-GFP/pgfp-rrnb.gb

11.7 KiB
text/plain
jtl
GenBank file of the integrative plasmid used to insert GFP in amyE locus.
LOCUS pGFP-rrnB 8009 bp ds-DNA circular 08-NOV-2018
DEFINITION .
ACCESSION pGFP
SOURCE Unknown.
ORGANISM Unknown Unclassified.
FEATURES Location/Qualifiers
CDS 6345..7052
/label="gfp' CDS"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature complement(7173..7259)
/label="ter misc_feature"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature complement(7078..7166)
/label="(null) misc_feature"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 199..1071
/label="bla CDS"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
CDS complement(2077..2892)
/label="spc CDS"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS complement(7375..7895)
/label="amyE' CDS"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS complement(5367..6029)
/label="cat CDS"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS complement(3968..5005)
/label="'amyE CDS"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
ORIGIN
1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
1321 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct
1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg
1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat
1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc
2041 agaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga
2101 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc
2161 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa
2221 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt
2281 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat
2341 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag
2401 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac
2461 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct
2521 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact
2581 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga
2641 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac
2701 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa
2761 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat
2821 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gttgattatg
2881 ttcatataaa gtttatattg atactcaatt tacttaccct agattggaca tatacttaaa
2941 ttactgttca ataaagctga ccgttagcgt ttaagtacat cctttcacaa tttgtctaca
3001 gattaataat tattctttat tatacagatc gatcctctag acctaggcct taagatctga
3061 tcatatgcat ccgcgggccc gggttaacgc gtaatccatg gatcaagaga caggatgagg
3121 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggtgc cctgaatgaa
3181 ctgcagaaag agctggtagt tggcgcactg ttcgaagaac tgccgatgtc cagtaagatt
3241 cttactatgc tggttgaacc ggatgctggt aaagctactt gggttgctgc ttctacttat
3301 ggtaccgata caactactgg tgaggaagtt aaaggagctc ttaaagaaat ccacttcagt
3361 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
3421 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
3481 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
3541 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
3601 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgacta
3661 agaaaatgcc gtcaaatccg ctcgccatga cttcactaac gatgcctttg aaaatcttca
3721 agttcttttc tactaattca aggcgtgtct caccaggttt ttggtttgct ccggcgcaaa
3781 tgcagacaat atcagcatcc ttgcagggta tgtttctctt tgatgtcttt ttgtttgtga
3841 agtatttcac atttatattg tgcaacactt cacaaacttt tgcaagagaa aagttttgtc
3901 tgatttatga acaaaaaaga aaccatcatt gatggtttct ttcggtaagt cccgtctagc
3961 cttgccctca atggggaaga gaaccgctta agcccgagtc attatataaa ccatttagca
4021 cgtaatcaaa gccaggctga ttctgaccgg gcacttgggc gctgccatta ttaaaaatca
4081 cttttgcgtt ggttgtatcc gtgtccgcag gcagcgtcag cgtgtaaatt ccgtctgcat
4141 ttttagtcat tggttttcca ggccaagatc cggtcaattc aattactcgg ctcccatcat
4201 gtttatagat ataagcattt acctggctcc aatgattcgg attttgatag ccgatggttt
4261 tggccgacgc tggatctctt ttaacaaaac tgtatttctc ggtcctcgtt acaccatcac
4321 tgttcgttcc ttttaacatg atggtgtatg ttttgccaaa ttggatctcc ttttccgatt
4381 gtgaattgat ctccatcctt aaacgcctgt cgtctggtcc attattgatt tgataaacgg
4441 cttttgttgt attcgcatct gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg
4501 ttacacctgt tttgtaattc tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat
4561 aaagcacagc tacagacctg gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa
4621 atgaacccgc tccagcttta ttgtcatacc tgccatcagg caattttgtt gccgtattga
4681 tagagacaga ggatgaacct gcatttgcca gcacaacgcc atgtgagccg cgctgattca
4741 taaatatctg gttgtttcca ttcgggttcg agagttcctc aggctgtcca gccatcacat
4801 tgtgaaatct attgaccgca gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc
4861 ctatttggct tttccccggg aacctcacac catttccgcc tccctcaggt ctggaaaaga
4921 aaagaggcgt actgcctgaa cgagaagcta tcaccgccca gcctaaacgg atatcatcat
4981 cgctcatcca tgtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
5041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
5101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
5161 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
5221 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggagctg
5281 taatataaaa accttcttca actaacgggg caggttagtg acattagaaa accgactgta
5341 aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat
5401 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc
5461 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata
5521 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc
5581 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc
5641 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca
5701 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa
5761 agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa
5821 gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat
5881 ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa
5941 gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca
6001 attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg
6061 cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa
6121 cataaatata tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg
6181 gtgctttagt tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa
6241 ggatttgagc gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg
6301 ccgatgataa gctgtcaaac atgagaattc gcggccgcgc tagcaaagga gaagaacttt
6361 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
6421 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
6481 gcactactgg aaaactacct gttccatggc caacacttgt cactactttg acctatggtg
6541 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
6601 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
6661 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
6721 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
6781 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
6841 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
6901 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
6961 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
7021 ggattacaca tggcatggat gagctctaca aataataagg ccggccaata aagatcctag
7081 gacgccgcca agccagctta aacccagctc aatgagctgg gttttttgtt tgttaaaaat
7141 gaagaagaaa ctgtgaagcg tatttaacta gtaaaatcta agacatatca tgatttataa
7201 aaacaaaaaa accttgcagg atatgcaagg cttctggatg acccgtacgg gattcgaact
7261 ccggatgtga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
7321 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatcgatca
7381 gaccagtttt taatttgtgt gtttccatgt gtccagtttg gaatactctt aacctcattg
7441 gaaatcgcgg cataatcact ggtggtatga ttgatgaccg cgtcaacaat gacctttatg
7501 ccatattctt cagcggctgc acacatttct ttaaattctt gttcagtacc taagtaacgg
7561 ttgccaattt gatacgatgt cggctgatac agccagtacc agttcgacat gcttttatct
7621 ccttgattcc cttcctttac ttggttaatc ggagatgtct gaatggctgt atatcctgca
7681 tcatgaatat ccttcatatt gtgttttaac gtattgaacg accaattcca tgcatgaaga
7741 atggttccgc ttttgatcga cggtgctgta agctcattcg atttgttcgc cgtttcagca
7801 ctcgcagccg ccggtcctgc cagaaccaaa tgaaacagca ataaaaatcc agcgaataac
7861 ggcagtaaag aggttttgaa tcgttttgca aacattcttg acactcctta tttgattttt
7921 tgaagactta cttcggagtc aaaaatccct cttacttcat tcttccgctt cctcctttca
7981 aaccgatgtg aagactggag aattttgtt
//