hg E.coli-Barcoding

Summary

Description:

This repository shows the work done in E.coli

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Latest Changes

Revision	Commit Message	Age	Author	Barcode
r7:e653989498f1	Corrected version	4 years and 4 months ago	jtl	default
r6:fb92a852d5e3	Vector for CRISPR barcoding	4 years and 4 months ago	jtl	default
r5:125790a5143c	Vector for lambda-red barcoding	4 years and 4 months ago	jtl	default
r4:9686c5bfac78	Sanger sequencing of barcoded region	4 years and 5 months ago	jtl	GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT default
r3:b77328a558d6	Strain was barcoded with BC383	4 years and 5 months ago	jtl	default
r2:aaf08b2520ba	Genotype correction: This strain was originally reported to be lacIQ, but it was later shown to be lacI+ (Baba 2006 ref. Supplementary Table 1)	4 years and 5 months ago	jtl	default
r1:633590c53f3e	Edited file README.md via cellrepo	4 years and 5 months ago	jtl	default
r0:14f127db0464	Added README file template	4 years and 5 months ago	cellrepo	default

README.md

E.coli-Barcoding

This repository shows the work done in E.coli

lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78

BC383

None

The strain was barcoded using Barcode 383.

https://www.pnas.org/content/97/12/6640
https://cgsc.biology.yale.edu/Strain.php?ID=64667
Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11