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LOCUS pEC-Vector 3777 bp ds-DNA circular 11-DEC-2019 |
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DEFINITION . |
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ACCESSION pTarget |
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SOURCE Unknown. |
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ORGANISM Unknown Unclassified. |
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FEATURES Location/Qualifiers |
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CDS complement(2531..2971) |
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/label="cat CDS" |
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/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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gene complement(2972..3190) |
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/label="cat gene" |
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/ApEinfo_revcolor=#75c6a9 |
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/ApEinfo_fwdcolor=#75c6a9 |
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misc_feature 2080..2522 |
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/label="left_homology" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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misc_feature 3301..3334 |
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/label="FRT" |
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/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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CDS complement(2972..3190) |
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/label="cat CDS" |
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/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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-35_signal complement(3280..3285) |
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/label="cat promoter (counter-clockwise); ACGTAA" |
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/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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rep_origin 151..532 |
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/label="rep_origin" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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promoter complement(1838..1866) |
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/label="amp prom" |
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/ApEinfo_revcolor=#ff9ccd |
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/ApEinfo_fwdcolor=#ff9ccd |
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-10_signal complement(3257..3262) |
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/label="cat promoter (counter-clockwise); CATATT" |
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/ApEinfo_revcolor=#9eafd2 |
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/ApEinfo_fwdcolor=#9eafd2 |
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misc_feature 1996..1997 |
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/label="Geneious type: origin" |
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/ApEinfo_revcolor=#c6c9d1 |
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/ApEinfo_fwdcolor=#c6c9d1 |
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marker complement(936..1796) |
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/label="amp marker" |
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/ApEinfo_revcolor=#f8d3a9 |
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/ApEinfo_fwdcolor=#f8d3a9 |
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misc_feature 3335..3777 |
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/label="right_homology" |
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/ApEinfo_revcolor=#faac61 |
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/ApEinfo_fwdcolor=#faac61 |
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misc_feature 3304..3331 |
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/label="overlap" |
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/ApEinfo_revcolor=#b4abac |
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/ApEinfo_fwdcolor=#b4abac |
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ORIGIN |
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1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg |
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61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca |
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121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta |
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181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac |
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241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg |
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301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat |
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361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt |
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421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag |
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481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac |
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541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac |
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601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc |
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661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt |
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721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc |
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781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg |
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841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca |
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901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca |
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961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag |
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1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac |
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1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc |
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1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct |
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1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc |
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1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg |
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1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc |
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1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat |
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1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag |
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1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat |
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1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg |
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1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca |
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1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga |
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1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc |
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1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata |
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1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg |
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1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc |
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1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag |
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2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc |
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2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt |
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2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga |
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2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc |
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2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc |
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2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa |
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2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca |
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2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg |
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2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca |
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2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca |
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2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt |
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2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga |
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2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca |
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2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg |
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2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata |
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2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg |
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3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa |
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3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg |
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3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt |
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3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg |
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3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc |
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3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac |
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3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg |
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3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga |
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3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt |
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3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt |
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3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct |
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3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc |
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3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc |
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// |
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