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Jonathan Tellechea (jtl) - 5 years ago 2019-12-11 15:19:44
j.tellechea-luzardo@newcastle.ac.uk
Vector for lambda-red barcoding
1 file changed with 123 insertions and 0 deletions:
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new file 100644
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LOCUS       pEC-Vector              3777 bp ds-DNA     circular     11-DEC-2019
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DEFINITION  .
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ACCESSION   pTarget                                                            
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SOURCE      Unknown.                                                           
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  ORGANISM  Unknown Unclassified.                                              
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FEATURES             Location/Qualifiers
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     CDS             complement(2531..2971)
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                     /label="cat CDS"
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                     /ApEinfo_revcolor=#c7b0e3
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                     /ApEinfo_fwdcolor=#c7b0e3
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     gene            complement(2972..3190)
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                     /label="cat gene"
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                     /ApEinfo_revcolor=#75c6a9
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                     /ApEinfo_fwdcolor=#75c6a9
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     misc_feature    2080..2522
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                     /label="left_homology"
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                     /ApEinfo_revcolor=#ffef86
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                     /ApEinfo_fwdcolor=#ffef86
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     misc_feature    3301..3334
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                     /label="FRT"
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                     /ApEinfo_revcolor=#c7b0e3
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                     /ApEinfo_fwdcolor=#c7b0e3
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     CDS             complement(2972..3190)
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                     /label="cat CDS"
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                     /ApEinfo_revcolor=#c7b0e3
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                     /ApEinfo_fwdcolor=#c7b0e3
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     -35_signal      complement(3280..3285)
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                     /label="cat promoter (counter-clockwise); ACGTAA"
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                     /ApEinfo_revcolor=#c7b0e3
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                     /ApEinfo_fwdcolor=#c7b0e3
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     rep_origin      151..532
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                     /label="rep_origin"
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                     /ApEinfo_revcolor=#d6b295
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                     /ApEinfo_fwdcolor=#d6b295
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     promoter        complement(1838..1866)
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                     /label="amp prom"
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                     /ApEinfo_revcolor=#ff9ccd
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                     /ApEinfo_fwdcolor=#ff9ccd
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     -10_signal      complement(3257..3262)
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                     /label="cat promoter (counter-clockwise); CATATT"
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                     /ApEinfo_revcolor=#9eafd2
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                     /ApEinfo_fwdcolor=#9eafd2
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     misc_feature    1996..1997
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                     /label="Geneious type: origin"
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                     /ApEinfo_revcolor=#c6c9d1
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                     /ApEinfo_fwdcolor=#c6c9d1
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     marker          complement(936..1796)
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                     /label="amp marker"
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                     /ApEinfo_revcolor=#f8d3a9
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                     /ApEinfo_fwdcolor=#f8d3a9
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     misc_feature    3335..3777
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                     /label="right_homology"
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                     /ApEinfo_revcolor=#faac61
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                     /ApEinfo_fwdcolor=#faac61
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     misc_feature    3304..3331
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                     /label="overlap"
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                     /ApEinfo_revcolor=#b4abac
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                     /ApEinfo_fwdcolor=#b4abac
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ORIGIN
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        1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg
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       61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
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      121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta
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      181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac
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      241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
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      301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
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      361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
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      421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
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      481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac
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      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
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      601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
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      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
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      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
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      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
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      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
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      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
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      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
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     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
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     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
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     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
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     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
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     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
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     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
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     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
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     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
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     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
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     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
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     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
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     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
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     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
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     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
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     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
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     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
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     1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
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     2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc
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     2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt
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     2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga
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     2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc
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     2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc
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     2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa
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     2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca
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     2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg
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     2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
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     2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
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     2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
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     2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
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     2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
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     2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
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     2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
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     2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
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     3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
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     3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
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     3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
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     3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
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     3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
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     3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac
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     3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg
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     3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga
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     3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt
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     3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt
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     3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct
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     3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc
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     3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc
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