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j.tellechea-luzardo@newcastle.ac.uk
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LOCUS pBS-CreLox-Vector 4456 bp ds-DNA circular 11-DEC-2019 |
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| 2 |
DEFINITION . |
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ACCESSION pTarget |
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SOURCE Unknown. |
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ORGANISM Unknown Unclassified. |
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FEATURES Location/Qualifiers |
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rep_origin join(4455..4456,1..681) |
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/label="ColE1 origin" |
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/ApEinfo_revcolor=#9eafd2 |
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/ApEinfo_fwdcolor=#9eafd2 |
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misc_feature 2399..2895 |
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/label="Right Homology" |
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/ApEinfo_revcolor=#d59687 |
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/ApEinfo_fwdcolor=#d59687 |
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misc_feature 2399..3398 |
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/label="Right Homology" |
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/ApEinfo_revcolor=#b7e6d7 |
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/ApEinfo_fwdcolor=#b7e6d7 |
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misc_feature 1887..1920 |
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/label="lox71" |
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/ApEinfo_revcolor=#faac61 |
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/ApEinfo_fwdcolor=#faac61 |
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misc_feature 1396..1880 |
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/label="Left Homology" |
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/ApEinfo_revcolor=#b7e6d7 |
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/ApEinfo_fwdcolor=#b7e6d7 |
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misc_feature 2365..2398 |
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/label="lox66" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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misc_feature 883..1880 |
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/label="Left homology" |
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/ApEinfo_revcolor=#ffef86 |
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/ApEinfo_fwdcolor=#ffef86 |
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misc_feature 1887..2398 |
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/label="lox71-zeocin-lox66" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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CDS 3565..4357 |
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/label="aadA_spec/strep_resistance" |
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/ApEinfo_revcolor=#d6b295 |
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/ApEinfo_fwdcolor=#d6b295 |
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CDS 75..663 |
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/label="pMB1" |
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/ApEinfo_revcolor=#75c6a9 |
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/ApEinfo_fwdcolor=#75c6a9 |
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misc_feature complement(3583..3603) |
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/label="SEQ and PCR" |
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/ApEinfo_revcolor=#75c6a9 |
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/ApEinfo_fwdcolor=#75c6a9 |
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ORIGIN |
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1 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag |
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61 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa |
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| 54 |
121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg |
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181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag |
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241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg |
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301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga |
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361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc |
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421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc |
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| 60 |
481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga |
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| 61 |
541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt |
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| 62 |
601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg |
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| 63 |
661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac |
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| 64 |
721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga |
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| 65 |
781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg |
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| 66 |
841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcaagaagat ttcacgcaat |
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| 67 |
901 ggtgtgccgc tgtatggcac aattgcggtg ctgatcggtt tggctgtcgg cgttgtactg |
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| 68 |
961 aactatatcg cgccgccgaa gatctttgtc tatgtataca gcgcaagcgt gctcccaggg |
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1021 atgattcctt ggtttatcat tttgatcagc catatcggat tcagaaaagc gaagggtgct |
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| 70 |
1081 gcattagata aacatccatt caaaatgccg tttgctcctt ttacaaacta tttgacgatt |
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1141 gctttcttgc tgatggttct ggttggcatg tggtttaacg atgatactcg tatctccctg |
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1201 attgtaggcg tgattttctt agcgcttgtg gtcattagtt attatgtgtt tgggattggc |
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1261 aaacgcacgc aggccaattt gacaaaaagc gaacaagcag ctgaataacg ataaaaaaga |
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1321 gacattcacg gatgtctctt tttttatttt tcgcgtatgg attttcccta ttttagcaag |
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1381 ctatagggta aagatagcag aaggaaaggt gttcataaaa tggatgttac actcggttat |
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1441 ttgcgcgaat cactttcgaa tcatctagaa aatgaagtct gccagcgtat ttgcaagaag |
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| 77 |
1501 atgctggcga agcgttatgc aaatgaagag gaatttgtaa aagacttaga tgataatgaa |
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| 78 |
1561 atgtcttttt tgaaccatgt attagaaaaa gaaattaagt atgcccagaa tgaacaggat |
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| 79 |
1621 caaaaacggg ccaaggaatt aaacgaggtg tatgaattac tgctttgaga aaggcgggta |
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| 80 |
1681 agaaatggga gcaattgaac gaagcggata tacatttcag ccggagttta gcgttgtccg |
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| 81 |
1741 tcaaaacgga gccatccatg tgtaccatca aggagaattt gtggaagaaa tcgaatttga |
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1801 atttaacggg gaatatcccg atcatgattt gattgaagag cttgtgaatc attattgttt |
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1861 tgagcatgag atttagagcc ACTAGTtacc gttcgtatag catacattat acgaagttat |
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1921 gtttttcatg aaagacttga tatggctttt tatatgtgtt actctacata cagaaaggag |
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1981 gaactaaata tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc |
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2041 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac |
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2101 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg |
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| 88 |
2161 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag |
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| 89 |
2221 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc |
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| 90 |
2281 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac |
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2341 ttcgtggccg aggagcagga ctgaataact tcgtatagca tacattatac gaacggtaat |
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| 92 |
2401 ttaggatgta atcaggccat actttttcag gatatccacg gtgatgtttt tggcttttgt |
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2461 gccggaatca tcgacattgg tgacaaagac ataagagcca tgctctgttt tgatgaatcc |
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2521 gacaaaccat cctaatccca tatcagtcag gcgggttccg gttttcccgt agagcgtgta |
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2581 atgatcgcct tcctcttgaa tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa |
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2641 tggaagctcc tctttagcca gtttctccaa aaaagtttct tgttccagcg gagatattgt |
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2701 gagcgagctt tgcagccaaa actggtcgat tcccccgctg atatcctcgt tgccgtaaga |
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2761 tagagtatga agccatgttt tcattctttc ctcgccgata tctctcgcta atgcctgata |
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2821 ataccagatt gcggattcgc gcatggccga gccaagcgta tggtcgcggt tccatgattc |
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2881 gaattcacgc ttgaccccgt cccatcgttt aacatcgtat tcatctctta cagctttgac |
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2941 ctgcaaaccg attaaagcgt tcacgacttt aaaggtggat tgcggagttt gtctttgatt |
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| 102 |
3001 tgcccgtttt ctgttataga caaatgtctg atccttttgt aaatcgtgaa gaatgaaggt |
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| 103 |
3061 tccatccgta tccttgaatt catcatcgac attcattttg ctgacattca agtgtttttc |
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| 104 |
3121 atgagcagat gatgctgcgt ggacggagaa gccgccgatc cctgcaatac tcagcacaag |
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| 105 |
3181 cacaacatat atccattttt tcattttgtt ctccccttcg ttttgattaa ctaccttttc |
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| 106 |
3241 tctctttcgt ttgttcttcc tttaaaaaat aatcaaaaag cgcttaaatt tggttttgca |
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3301 aacaaatact gttactgggt ttatagtcgg aagataacgt gaaaatagag ggtaaaaagg |
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3361 aaggcagggt aagcggatga atgagcagat tccacatggt cgacctgcag aagcttagat |
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3421 ctattaccct gttatcccta ctcgagttca tgtgcagctc catcagcaaa aggggatgat |
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3481 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg |
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3541 tcatcagcgg tggagtgcaa tgtcatgagg gaagcggtga tcgccgaagt atcgactcaa |
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3601 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat |
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| 113 |
3661 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt |
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| 114 |
3721 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa |
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| 115 |
3781 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg |
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| 116 |
3841 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg |
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3901 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct |
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| 118 |
3961 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa |
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4021 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta |
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| 120 |
4081 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc |
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| 121 |
4141 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca |
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| 122 |
4201 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt |
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| 123 |
4261 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac |
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4321 gtgaaaggcg agatcaccaa ggtagtcggc aaataagatg ccgctcgcca gtcgattggc |
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4381 tgagctcatg aagttcctat tccgaagttc cgcgaacgcg taaaggatct aggtgaagat |
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4441 cctttttgat aatctc |
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// |
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No newline at end of file |
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