LOCUS       pBS-CreLox-Vector       4456 bp ds-DNA     circular     11-DEC-2019
DEFINITION  .
ACCESSION   pTarget                                                            
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     rep_origin      join(4455..4456,1..681)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2399..2895
                     /label="Right Homology"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2399..3398
                     /label="Right Homology"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1887..1920
                     /label="lox71"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1396..1880
                     /label="Left Homology"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2365..2398
                     /label="lox66"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    883..1880
                     /label="Left homology"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1887..2398
                     /label="lox71-zeocin-lox66"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3565..4357
                     /label="aadA_spec/strep_resistance"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             75..663
                     /label="pMB1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(3583..3603)
                     /label="SEQ and PCR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
       61 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
      241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcaagaagat ttcacgcaat
      901 ggtgtgccgc tgtatggcac aattgcggtg ctgatcggtt tggctgtcgg cgttgtactg
      961 aactatatcg cgccgccgaa gatctttgtc tatgtataca gcgcaagcgt gctcccaggg
     1021 atgattcctt ggtttatcat tttgatcagc catatcggat tcagaaaagc gaagggtgct
     1081 gcattagata aacatccatt caaaatgccg tttgctcctt ttacaaacta tttgacgatt
     1141 gctttcttgc tgatggttct ggttggcatg tggtttaacg atgatactcg tatctccctg
     1201 attgtaggcg tgattttctt agcgcttgtg gtcattagtt attatgtgtt tgggattggc
     1261 aaacgcacgc aggccaattt gacaaaaagc gaacaagcag ctgaataacg ataaaaaaga
     1321 gacattcacg gatgtctctt tttttatttt tcgcgtatgg attttcccta ttttagcaag
     1381 ctatagggta aagatagcag aaggaaaggt gttcataaaa tggatgttac actcggttat
     1441 ttgcgcgaat cactttcgaa tcatctagaa aatgaagtct gccagcgtat ttgcaagaag
     1501 atgctggcga agcgttatgc aaatgaagag gaatttgtaa aagacttaga tgataatgaa
     1561 atgtcttttt tgaaccatgt attagaaaaa gaaattaagt atgcccagaa tgaacaggat
     1621 caaaaacggg ccaaggaatt aaacgaggtg tatgaattac tgctttgaga aaggcgggta
     1681 agaaatggga gcaattgaac gaagcggata tacatttcag ccggagttta gcgttgtccg
     1741 tcaaaacgga gccatccatg tgtaccatca aggagaattt gtggaagaaa tcgaatttga
     1801 atttaacggg gaatatcccg atcatgattt gattgaagag cttgtgaatc attattgttt
     1861 tgagcatgag atttagagcc ACTAGTtacc gttcgtatag catacattat acgaagttat
     1921 gtttttcatg aaagacttga tatggctttt tatatgtgtt actctacata cagaaaggag
     1981 gaactaaata tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     2041 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     2101 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     2161 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     2221 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     2281 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     2341 ttcgtggccg aggagcagga ctgaataact tcgtatagca tacattatac gaacggtaat
     2401 ttaggatgta atcaggccat actttttcag gatatccacg gtgatgtttt tggcttttgt
     2461 gccggaatca tcgacattgg tgacaaagac ataagagcca tgctctgttt tgatgaatcc
     2521 gacaaaccat cctaatccca tatcagtcag gcgggttccg gttttcccgt agagcgtgta
     2581 atgatcgcct tcctcttgaa tcatcatgcg cttgacgatt ttcatcaccg gcttgtcaaa
     2641 tggaagctcc tctttagcca gtttctccaa aaaagtttct tgttccagcg gagatattgt
     2701 gagcgagctt tgcagccaaa actggtcgat tcccccgctg atatcctcgt tgccgtaaga
     2761 tagagtatga agccatgttt tcattctttc ctcgccgata tctctcgcta atgcctgata
     2821 ataccagatt gcggattcgc gcatggccga gccaagcgta tggtcgcggt tccatgattc
     2881 gaattcacgc ttgaccccgt cccatcgttt aacatcgtat tcatctctta cagctttgac
     2941 ctgcaaaccg attaaagcgt tcacgacttt aaaggtggat tgcggagttt gtctttgatt
     3001 tgcccgtttt ctgttataga caaatgtctg atccttttgt aaatcgtgaa gaatgaaggt
     3061 tccatccgta tccttgaatt catcatcgac attcattttg ctgacattca agtgtttttc
     3121 atgagcagat gatgctgcgt ggacggagaa gccgccgatc cctgcaatac tcagcacaag
     3181 cacaacatat atccattttt tcattttgtt ctccccttcg ttttgattaa ctaccttttc
     3241 tctctttcgt ttgttcttcc tttaaaaaat aatcaaaaag cgcttaaatt tggttttgca
     3301 aacaaatact gttactgggt ttatagtcgg aagataacgt gaaaatagag ggtaaaaagg
     3361 aaggcagggt aagcggatga atgagcagat tccacatggt cgacctgcag aagcttagat
     3421 ctattaccct gttatcccta ctcgagttca tgtgcagctc catcagcaaa aggggatgat
     3481 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg
     3541 tcatcagcgg tggagtgcaa tgtcatgagg gaagcggtga tcgccgaagt atcgactcaa
     3601 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     3661 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     3721 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     3781 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     3841 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     3901 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     3961 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     4021 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     4081 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     4141 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     4201 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     4261 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     4321 gtgaaaggcg agatcaccaa ggtagtcggc aaataagatg ccgctcgcca gtcgattggc
     4381 tgagctcatg aagttcctat tccgaagttc cgcgaacgcg taaaggatct aggtgaagat
     4441 cctttttgat aatctc
//