LOCUS       pEC-Vector              3777 bp ds-DNA     circular     11-DEC-2019
DEFINITION  .
ACCESSION   pTarget                                                            
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     CDS             complement(2531..2971)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            complement(2972..3190)
                     /label="cat gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2080..2522
                     /label="left_homology"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3301..3334
                     /label="FRT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(2972..3190)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -35_signal      complement(3280..3285)
                     /label="cat promoter (counter-clockwise); ACGTAA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      151..532
                     /label="rep_origin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        complement(1838..1866)
                     /label="amp prom"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     -10_signal      complement(3257..3262)
                     /label="cat promoter (counter-clockwise); CATATT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1996..1997
                     /label="Geneious type: origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     marker          complement(936..1796)
                     /label="amp marker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3335..3777
                     /label="right_homology"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3304..3331
                     /label="overlap"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg
       61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta
      181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac
      241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
      301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
      361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
      421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
      481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc
     2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt
     2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga
     2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc
     2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc
     2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa
     2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca
     2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg
     2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac
     3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg
     3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga
     3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt
     3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt
     3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct
     3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc
     3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc
//