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Jonathan Tellechea (jtl) - 5 years ago 2019-12-11 15:56:21
j.tellechea-luzardo@newcastle.ac.uk
Corrected version
1 file changed with 58 insertions and 57 deletions:
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1
 
LOCUS       pEC-Vector              3777 bp ds-DNA     circular     11-DEC-2019
1
 
LOCUS       pEC-Vector              3811 bp ds-DNA     circular     11-DEC-2019
2 2
 
DEFINITION  .
3 3
 
ACCESSION   pTarget                                                            
4 4
 
SOURCE      Unknown.                                                           
5 5
 
  ORGANISM  Unknown Unclassified.                                              
6 6
 
FEATURES             Location/Qualifiers
7
 
     CDS             complement(2531..2971)
8
 
                     /label="cat CDS"
9
 
                     /ApEinfo_revcolor=#c7b0e3
10
 
                     /ApEinfo_fwdcolor=#c7b0e3
11
 
     gene            complement(2972..3190)
12
 
                     /label="cat gene"
13
 
                     /ApEinfo_revcolor=#75c6a9
14
 
                     /ApEinfo_fwdcolor=#75c6a9
15 7
 
     misc_feature    2080..2522
16 8
 
                     /label="left_homology"
17 9
 
                     /ApEinfo_revcolor=#ffef86
18 10
 
                     /ApEinfo_fwdcolor=#ffef86
19
 
     misc_feature    3301..3334
11
 
     misc_feature    3369..3811
12
 
                     /label="right_homology"
13
 
                     /ApEinfo_revcolor=#faac61
14
 
                     /ApEinfo_fwdcolor=#faac61
15
 
     misc_feature    3335..3368
20 16
 
                     /label="FRT"
21 17
 
                     /ApEinfo_revcolor=#c7b0e3
22 18
 
                     /ApEinfo_fwdcolor=#c7b0e3
23
 
     CDS             complement(2972..3190)
19
 
     -10_signal      complement(3291..3296)
20
 
                     /label="cat promoter (counter-clockwise); CATATT"
21
 
                     /ApEinfo_revcolor=#9eafd2
22
 
                     /ApEinfo_fwdcolor=#9eafd2
23
 
     CDS             complement(2565..3005)
24 24
 
                     /label="cat CDS"
25 25
 
                     /ApEinfo_revcolor=#c7b0e3
26 26
 
                     /ApEinfo_fwdcolor=#c7b0e3
27
 
     -35_signal      complement(3280..3285)
27
 
     gene            complement(3006..3224)
28
 
                     /label="cat gene"
29
 
                     /ApEinfo_revcolor=#75c6a9
30
 
                     /ApEinfo_fwdcolor=#75c6a9
31
 
     marker          complement(936..1796)
32
 
                     /label="amp marker"
33
 
                     /ApEinfo_revcolor=#f8d3a9
34
 
                     /ApEinfo_fwdcolor=#f8d3a9
35
 
     promoter        complement(1838..1866)
36
 
                     /label="amp prom"
37
 
                     /ApEinfo_revcolor=#ff9ccd
38
 
                     /ApEinfo_fwdcolor=#ff9ccd
39
 
     misc_feature    2530..2563
40
 
                     /label="FRT"
41
 
                     /ApEinfo_revcolor=#c7b0e3
42
 
                     /ApEinfo_fwdcolor=#c7b0e3
43
 
     -35_signal      complement(3314..3319)
28 44
 
                     /label="cat promoter (counter-clockwise); ACGTAA"
29 45
 
                     /ApEinfo_revcolor=#c7b0e3
30 46
 
                     /ApEinfo_fwdcolor=#c7b0e3
47
 
     misc_feature    1996..1997
48
 
                     /label="Geneious type: origin"
49
 
                     /ApEinfo_revcolor=#c6c9d1
50
 
                     /ApEinfo_fwdcolor=#c6c9d1
51
 
     CDS             complement(3006..3224)
52
 
                     /label="cat CDS"
53
 
                     /ApEinfo_revcolor=#c7b0e3
54
 
                     /ApEinfo_fwdcolor=#c7b0e3
31 55
 
     rep_origin      151..532
32 56
 
                     /label="rep_origin"
33 57
 
                     /ApEinfo_revcolor=#d6b295
34 58
 
                     /ApEinfo_fwdcolor=#d6b295
35
 
     promoter        complement(1838..1866)
36
 
                     /label="amp prom"
37
 
                     /ApEinfo_revcolor=#ff9ccd
38
 
                     /ApEinfo_fwdcolor=#ff9ccd
39
 
     -10_signal      complement(3257..3262)
40
 
                     /label="cat promoter (counter-clockwise); CATATT"
41
 
                     /ApEinfo_revcolor=#9eafd2
42
 
                     /ApEinfo_fwdcolor=#9eafd2
43
 
     misc_feature    1996..1997
44
 
                     /label="Geneious type: origin"
45
 
                     /ApEinfo_revcolor=#c6c9d1
46
 
                     /ApEinfo_fwdcolor=#c6c9d1
47
 
     marker          complement(936..1796)
48
 
                     /label="amp marker"
49
 
                     /ApEinfo_revcolor=#f8d3a9
50
 
                     /ApEinfo_fwdcolor=#f8d3a9
51
 
     misc_feature    3335..3777
52
 
                     /label="right_homology"
53
 
                     /ApEinfo_revcolor=#faac61
54
 
                     /ApEinfo_fwdcolor=#faac61
55
 
     misc_feature    3304..3331
56
 
                     /label="overlap"
57
 
                     /ApEinfo_revcolor=#b4abac
58
 
                     /ApEinfo_fwdcolor=#b4abac
59 59
 
ORIGIN
60 60
 
        1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg
61 61
 
       61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
62 62
 
      121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta
63 63
 
      181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac
64 64
 
      241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
65 65
 
      301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
66 66
 
      361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
67 67
 
      421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
68 68
 
      481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac
69 69
 
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
70 70
 
      601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
71 71
 
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
72 72
 
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
73 73
 
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
74 74
 
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
75 75
 
      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
76 76
 
      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
77 77
 
     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
78 78
 
     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
79 79
 
     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
80 80
 
     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
81 81
 
     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
82 82
 
     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
83 83
 
     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
84 84
 
     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
85 85
 
     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
86 86
 
     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
87 87
 
     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
88 88
 
     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
89 89
 
     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
90 90
 
     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
91 91
 
     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
92 92
 
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
93 93
 
     1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
94 94
 
     2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc
95 95
 
     2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt
96 96
 
     2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga
97 97
 
     2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc
98 98
 
     2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc
99 99
 
     2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa
100 100
 
     2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca
101 101
 
     2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg
102
 
     2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
103
 
     2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
104
 
     2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
105
 
     2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
106
 
     2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
107
 
     2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
108
 
     2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
109
 
     2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
110
 
     3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
111
 
     3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
112
 
     3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
113
 
     3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
114
 
     3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
115
 
     3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac
116
 
     3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg
117
 
     3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga
118
 
     3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt
119
 
     3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt
120
 
     3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct
121
 
     3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc
122
 
     3721 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatc
102
 
     2521 cgtGGATCCg aagttcctat tctctagaaa gtataggaac ttcattacgc cccgccctgc
103
 
     2581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
104
 
     2641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
105
 
     2701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
106
 
     2761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
107
 
     2821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
108
 
     2881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
109
 
     2941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
110
 
     3001 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
111
 
     3061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
112
 
     3121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
113
 
     3181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
114
 
     3241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
115
 
     3301 aagttggaac ctcttacgtg ccgatcaacg tctcgaagtt cctattctct agaaagtata
116
 
     3361 ggaacttcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg
117
 
     3421 gaagccctat ttcttttatg gaatcagcag gctggaacct tgcgtcgccc ggctttccag
118
 
     3481 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac
119
 
     3541 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc
120
 
     3601 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt
121
 
     3661 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa
122
 
     3721 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta
123
 
     3781 caccacgcgc actttcttac cgccggtgat c
123 124
 
//
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