LOCUS       pEC-Vector              3811 bp ds-DNA     circular     11-DEC-2019
DEFINITION  .
ACCESSION   pTarget                                                            
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     misc_feature    2080..2522
                     /label="left_homology"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3369..3811
                     /label="right_homology"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3335..3368
                     /label="FRT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      complement(3291..3296)
                     /label="cat promoter (counter-clockwise); CATATT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2565..3005)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            complement(3006..3224)
                     /label="cat gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     marker          complement(936..1796)
                     /label="amp marker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        complement(1838..1866)
                     /label="amp prom"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2530..2563
                     /label="FRT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -35_signal      complement(3314..3319)
                     /label="cat promoter (counter-clockwise); ACGTAA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1996..1997
                     /label="Geneious type: origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(3006..3224)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      151..532
                     /label="rep_origin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg
       61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      121 gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata gtacgtacta
      181 agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac
      241 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
      301 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
      361 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
      421 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
      481 cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc ggctggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg tagtcaaatt caccacgccc
     2101 tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt
     2161 gtaatgccag caagagattt catatttggg agagcatcat gctggaacta ctttttgtga
     2221 ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc
     2281 tggttgtggc gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc
     2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa
     2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca
     2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg
     2521 cgtGGATCCg aagttcctat tctctagaaa gtataggaac ttcattacgc cccgccctgc
     2581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     2641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     2821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     2941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     3001 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     3061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     3121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     3181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     3241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     3301 aagttggaac ctcttacgtg ccgatcaacg tctcgaagtt cctattctct agaaagtata
     3361 ggaacttcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg
     3421 gaagccctat ttcttttatg gaatcagcag gctggaacct tgcgtcgccc ggctttccag
     3481 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac
     3541 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc
     3601 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt
     3661 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa
     3721 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta
     3781 caccacgcgc actttcttac cgccggtgat c
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