1 |
LOCUS pEC-Vector |
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1 |
LOCUS pEC-Vector 3811 bp ds-DNA circular 11-DEC-2019 |
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2 | 2 |
DEFINITION . |
3 | 3 |
ACCESSION pTarget |
4 | 4 |
SOURCE Unknown. |
5 | 5 |
ORGANISM Unknown Unclassified. |
6 | 6 |
FEATURES Location/Qualifiers |
7 |
CDS complement(2531..2971) |
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8 |
/label="cat CDS" |
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9 |
/ApEinfo_revcolor=#c7b0e3 |
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10 |
/ApEinfo_fwdcolor=#c7b0e3 |
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11 |
gene complement(2972..3190) |
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12 |
/label="cat gene" |
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13 |
/ApEinfo_revcolor=#75c6a9 |
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14 |
/ApEinfo_fwdcolor=#75c6a9 |
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15 | 7 |
misc_feature 2080..2522 |
16 | 8 |
/label="left_homology" |
17 | 9 |
/ApEinfo_revcolor=#ffef86 |
18 | 10 |
/ApEinfo_fwdcolor=#ffef86 |
19 |
misc_feature |
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11 |
misc_feature 3369..3811 |
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12 |
/label="right_homology" |
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13 |
/ApEinfo_revcolor=#faac61 |
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14 |
/ApEinfo_fwdcolor=#faac61 |
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15 |
misc_feature 3335..3368 |
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20 | 16 |
/label="FRT" |
21 | 17 |
/ApEinfo_revcolor=#c7b0e3 |
22 | 18 |
/ApEinfo_fwdcolor=#c7b0e3 |
23 |
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19 |
-10_signal complement(3291..3296) |
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20 |
/label="cat promoter (counter-clockwise); CATATT" |
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21 |
/ApEinfo_revcolor=#9eafd2 |
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22 |
/ApEinfo_fwdcolor=#9eafd2 |
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23 |
CDS complement(2565..3005) |
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24 | 24 |
/label="cat CDS" |
25 | 25 |
/ApEinfo_revcolor=#c7b0e3 |
26 | 26 |
/ApEinfo_fwdcolor=#c7b0e3 |
27 |
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27 |
gene complement(3006..3224) |
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28 |
/label="cat gene" |
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29 |
/ApEinfo_revcolor=#75c6a9 |
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30 |
/ApEinfo_fwdcolor=#75c6a9 |
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31 |
marker complement(936..1796) |
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/label="amp marker" |
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33 |
/ApEinfo_revcolor=#f8d3a9 |
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34 |
/ApEinfo_fwdcolor=#f8d3a9 |
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35 |
promoter complement(1838..1866) |
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/label="amp prom" |
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37 |
/ApEinfo_revcolor=#ff9ccd |
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38 |
/ApEinfo_fwdcolor=#ff9ccd |
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39 |
misc_feature 2530..2563 |
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/label="FRT" |
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41 |
/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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43 |
-35_signal complement(3314..3319) |
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28 | 44 |
/label="cat promoter (counter-clockwise); ACGTAA" |
29 | 45 |
/ApEinfo_revcolor=#c7b0e3 |
30 | 46 |
/ApEinfo_fwdcolor=#c7b0e3 |
47 |
misc_feature 1996..1997 |
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/label="Geneious type: origin" |
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/ApEinfo_revcolor=#c6c9d1 |
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50 |
/ApEinfo_fwdcolor=#c6c9d1 |
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CDS complement(3006..3224) |
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52 |
/label="cat CDS" |
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53 |
/ApEinfo_revcolor=#c7b0e3 |
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/ApEinfo_fwdcolor=#c7b0e3 |
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31 | 55 |
rep_origin 151..532 |
32 | 56 |
/label="rep_origin" |
33 | 57 |
/ApEinfo_revcolor=#d6b295 |
34 | 58 |
/ApEinfo_fwdcolor=#d6b295 |
35 |
promoter complement(1838..1866) |
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36 |
/label="amp prom" |
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37 |
/ApEinfo_revcolor=#ff9ccd |
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38 |
/ApEinfo_fwdcolor=#ff9ccd |
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39 |
-10_signal complement(3257..3262) |
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40 |
/label="cat promoter (counter-clockwise); CATATT" |
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41 |
/ApEinfo_revcolor=#9eafd2 |
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42 |
/ApEinfo_fwdcolor=#9eafd2 |
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43 |
misc_feature 1996..1997 |
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44 |
/label="Geneious type: origin" |
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45 |
/ApEinfo_revcolor=#c6c9d1 |
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46 |
/ApEinfo_fwdcolor=#c6c9d1 |
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47 |
marker complement(936..1796) |
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48 |
/label="amp marker" |
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49 |
/ApEinfo_revcolor=#f8d3a9 |
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50 |
/ApEinfo_fwdcolor=#f8d3a9 |
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misc_feature 3335..3777 |
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/label="right_homology" |
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/ApEinfo_revcolor=#faac61 |
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54 |
/ApEinfo_fwdcolor=#faac61 |
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misc_feature 3304..3331 |
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/label="overlap" |
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57 |
/ApEinfo_revcolor=#b4abac |
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58 |
/ApEinfo_fwdcolor=#b4abac |
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59 | 59 |
ORIGIN |
60 | 60 |
1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc gctgcgctcg |
61 | 61 |
61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca |
... |
@@ -99,25 +99,26 @@ ORIGIN |
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99 | 99 |
2341 tgccgtggtt actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa |
100 | 100 |
2401 aagtgccgaa atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca |
101 | 101 |
2461 caacctaagg ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg |
102 |
2521 cgtGGATCCa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca |
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103 |
2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca |
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104 |
2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt |
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105 |
2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga |
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106 |
2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca |
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107 |
2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg |
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108 |
2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata |
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109 |
2941 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg |
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110 |
3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa |
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111 |
3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg |
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112 |
3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt |
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113 |
3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg |
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114 |
3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc |
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115 |
3301 gaagttccta ttctctagaa agtataggaa cttcgtgctg taccctacat acagccgaac |
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116 |
3361 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg |
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117 |
3421 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga |
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118 |
3481 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt |
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119 |
3541 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt |
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120 |
3601 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct |
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121 |
3661 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc |
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122 |
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102 |
2521 cgtGGATCCg aagttcctat tctctagaaa gtataggaac ttcattacgc cccgccctgc |
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103 |
2581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa |
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104 |
2641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat |
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105 |
2701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa |
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106 |
2761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta |
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107 |
2821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac |
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108 |
2881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg |
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109 |
2941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc |
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110 |
3001 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa |
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111 |
3061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc |
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112 |
3121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat |
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113 |
3181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct |
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114 |
3241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga |
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115 |
3301 aagttggaac ctcttacgtg ccgatcaacg tctcgaagtt cctattctct agaaagtata |
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116 |
3361 ggaacttcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg |
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117 |
3421 gaagccctat ttcttttatg gaatcagcag gctggaacct tgcgtcgccc ggctttccag |
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118 |
3481 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac |
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119 |
3541 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc |
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120 |
3601 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt |
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121 |
3661 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa |
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122 |
3721 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta |
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123 |
3781 caccacgcgc actttcttac cgccggtgat c |
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123 | 124 |
// |
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