LOCUS       pEC-CRISPR-Vector       3178 bp ds-DNA     circular     11-DEC-2019
DEFINITION  .
SOURCE      Cloning vector pUC19                                               
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;        
            vectors.
FEATURES             Location/Qualifiers
     misc_RNA        complement(1359..1911)
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            complement(2118..2978)
                     /label="bla gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1123..1162
                     /label="spy-tem"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -35_signal      complement(3043..3048)
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1359..1947)
                     /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -10_signal      complement(3022..3027)
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -35_signal      1765..1770
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    535..977
                     /label="right_homology"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1081..1122
                     /label="SP_scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_RNA        1801..1908
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -35_signal      complement(1942..1947)
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     -10_signal      1787..1792
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -10_signal      complement(1921..1926)
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    84..526
                     /label="left_homology"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1026..1054
                     /label="pJ23119"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     sig_peptide     complement(2910..2978)
                     /label="bla sig_peptide"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2118..2978)
                     /label="bla CDS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgtagtca aattcaccac gccctgcgca ccgtcgcggg
      121 gcgttttgct gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag
      181 atttcatatt tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat
      241 gctgatggtc accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc
      301 cattatgttc ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact
      361 ggcgattgcg gtggtgtggg ttattaaggc gattaaagca ccaaaagtgc cgaaatatca
      421 gcgttatgac cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat
      481 ccttagaaca aaataggaat tgataatcaa atctgtcact attgcgtGCA TGCagtgctg
      541 taccctacat acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct
      601 tttatggaat cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac
      661 gctgcgcatc cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc
      721 tggcaatcaa agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat
      781 agccttgcag ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt
      841 tcacaccggt aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca
      901 ggcaatccag cgaccgttcc caggtgtctc tgcccacgga atcgtacacc acgcgcactt
      961 tcttaccgcc ggtgatcttc tccttacgca tctgtgcggt atttcacacc gcatatgctg
     1021 gatccttgac agctagctca gtcctaggta taatactagt CAGCACGATG AATCTGTTAG
     1081 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1141 ggcaccgagt cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta
     1201 ttaccctgtt atccctactc gagttcatgt gcagctccag ctcactcaaa ggcggtaata
     1261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//